Department of Applied Molecular Chemistry, Graduate School of Industrial Technology, Nihon University, 1-2-1, Izumichou, Narashino, Chiba 275-8575, Japan.
J Biosci Bioeng. 2011 Dec;112(6):602-5. doi: 10.1016/j.jbiosc.2011.08.008. Epub 2011 Sep 8.
Photosynthetic bacteria produce hydrogen from lactate and acetate that are products of hydrogen producing bacteria in the dark. Thus, their coculture is a promising method for hydrogen production. However, the hydrogen production yield from acetate of Rhodobacter sphaeroides RV, which has been shown to possess the highest yield and hydrogen production rate, is low as compared to that from lactate. Photosynthetic bacteria that produce hydrogen from acetate as well as lactate were screened from lakes and swamps in the Tokyo and Chiba areas in Japan. Seventy-six strains of photosynthetic bacteria were obtained and the analysis of their 16S rRNA gene sequences revealed that they belong to R. sphaeroides. Among the isolated bacteria, R. sphaeroides HJ produced the highest amount of hydrogen from acetate and lactate. The HJ strain produced a 2300±93ml/L-broth of hydrogen from 75mM acetate consumed during for 120h of fermentation. The amount of hydrogen and the yield from acetate were 1.9 and 2.1 times higher, respectively, than those of R. sphaeroides RV. The amount and yield of hydrogen, produced by R. sphaeroides HJ from lactate were similar to those produced by R. sphaeroides RV. Since the amount and yield of produced hydrogen by the HJ strain were similar regardless of the substrate (acetate or lactate), its metabolic pathway could have a key to increasing hydrogen production from acetate.
光合细菌可以在黑暗中利用产氢细菌产生的乳酸盐和醋酸盐来生产氢气。因此，它们的共培养是一种很有前途的产氢方法。然而，与乳酸盐相比，已经证明具有最高产氢率和产氢速率的球形红杆菌 RV 从醋酸盐中生产的氢气产量较低。从日本东京和千叶地区的湖泊和沼泽中筛选出既能从醋酸盐又能从乳酸盐生产氢气的光合细菌。获得了 76 株光合细菌，并对其 16S rRNA 基因序列进行了分析，结果表明它们属于球形红杆菌。在分离出的细菌中，HJ 菌株从醋酸盐和乳酸盐中产生的氢气量最高。HJ 菌株在 120 小时的发酵过程中消耗了 75mM 醋酸盐，产生了 2300±93ml/L 的发酵液氢气。从醋酸盐产生的氢气量和产率分别比 RV 菌株高 1.9 倍和 2.1 倍。HJ 菌株从乳酸盐生产的氢气量和产率与 RV 菌株相似。由于 HJ 菌株产生的氢气量和产率与底物（醋酸盐或乳酸盐）无关，因此其代谢途径可能是增加醋酸盐产氢的关键。
