约旦不同常见即食食品中单核细胞增生李斯特菌及其他李斯特菌属污染情况的调查
Survey of Listeria monocytogenes and other Listeria sp. contamination in different common ready-to-eat food products in Jordan.
作者信息
Awaisheh S S
机构信息
Food Microbiology and Safety Laboratory, Department of Food Science, Faculty of Agriculture, Mu'tah University, Karak, 71610, Jordan.
出版信息
Pak J Biol Sci. 2009 Dec 1;12(23):1491-7. doi: 10.3923/pjbs.2009.1491.1497.
Incidence and contamination levels of different Listeria monocytogenes serovars and different Listeria sp. in 360 samples of common ready-to-eat food products in Jordan were investigated. The presence of L. monocytogenes was determined using EN ISO protocol and confirmed using PCR technique. Five Listeria sp.: L. monocytogenes, L. innocua, L. ivanovvi, L. welshimeri and L. seeligeri were isolated. L. monocytogenes was isolated from 19 samples (5.3%), from 6 RTE dairy samples; 6 RTE vegetables samples and 4 traditional dishes samples and 3 miscellaneous samples. L. innocua and L. ivanovvi were the most and least frequently isolated species, 24 and 3 samples, respectively. L. welshimeri was isolated from 8 samples and L. seeligeri from 7 samples. The contamination levels of L. monocytogenes were found to be < or = 100 CFU g(-1) in 84.2% (16 samples) of the positive samples. Only 15.8% (3 samples; 1 vegetable, 1 traditional dish and 1 miscellaneous samples) of the positive samples were found with counts >100 CFU g(-1). L. monocytogenes strains isolated fell into 2 serotype, 1 and 4 and to 5 different serovars, 1/2a, 1/2b, 1/2c, 4a, 4c.
对约旦360份即食食品常见样本中不同单核细胞增生李斯特菌血清型和不同李斯特菌属的发病率及污染水平进行了调查。使用EN ISO协议确定单核细胞增生李斯特菌的存在，并采用PCR技术进行确认。分离出了5种李斯特菌属：单核细胞增生李斯特菌、无害李斯特菌、伊氏李斯特菌、威氏李斯特菌和斯氏李斯特菌。从19份样本（5.3%）中分离出了单核细胞增生李斯特菌，其中6份为即食乳制品样本；6份即食蔬菜样本、4份传统菜肴样本和3份其他样本。无害李斯特菌和伊氏李斯特菌是分离频率最高和最低的菌种，分别为24份和3份样本。从8份样本中分离出了威氏李斯特菌，从7份样本中分离出了斯氏李斯特菌。在84.2%（16份样本）的阳性样本中，单核细胞增生李斯特菌的污染水平被发现≤100 CFU g(-1)。仅15.8%（3份样本；1份蔬菜样本、1份传统菜肴样本和1份其他样本）的阳性样本计数>100 CFU g(-1)。分离出的单核细胞增生李斯特菌菌株分为2个血清型，1型和4型，以及5个不同的血清变种，1/2a、1/2b、1/2c、4a、4c。
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