Xie Hui, Huang Hui-Cong, Yang Yi-Rong, He Qiu-Xiang, Zhu Qi-Jian, Chen Jian-Ou
Department of Urology, The First Hospital Affiliated to Wenzhou Medical College, Wenzhou, Zhejiang 325000, China.
Zhonghua Nan Ke Xue. 2010 Jan;16(1):40-3.
To investigate the role of bacteria in the etiology of chronic prostatitis.
A total of 162 complete prostate specimens were obtained at autopsy from organ donors (aged 20 -38 yr) who died of non-prostatic diseases. Each of the samples from the peripheral zone of the prostate was divided into two parts, one for routine pathological examination and immunohistochemical studies of interleukin (IL)-1beta, tumor necrosis factor-alpha (TNF-alpha) and the nerve growth factor (NGF), and the other for PCR assay to detect the bacterial 16S rRNA gene (16S rDNA).
Fifty-one (31.5%) of the total specimens presented pathological changes of chronic prostatitis, of which 44 had mild focal stromal, 5 mild focal stromal and periglandular and 2 mild focal periglandular inflammation. The positive rate of 16S rDNA was 19.1% (31/162), 51.0% (26/51) in the chronic prostatitis and 4.5% (5/111) in the non-prostatitis specimens (chi2 = 29.783, P < 0.01). In the specimens with chronic prostatitis, the expressions of IL-1beta, TNF-alpha and NGF were significantly higher in the 16S rDNA positive than in the 16S rDNA negative group (P < 0.01).
Bacterial inflammation may play an important role in the etiology of chronic prostatitis.
探讨细菌在慢性前列腺炎病因学中的作用。
从死于非前列腺疾病的器官捐献者（年龄20 - 38岁）尸检中获取162份完整的前列腺标本。前列腺外周区的每个样本均分为两部分，一部分用于白细胞介素（IL）-1β、肿瘤坏死因子-α（TNF-α）和神经生长因子（NGF）的常规病理检查及免疫组化研究，另一部分用于聚合酶链反应（PCR）检测细菌16S核糖体RNA基因（16S rDNA）。
全部标本中有51份（31.5%）呈现慢性前列腺炎的病理改变，其中44份有轻度局灶性间质炎症，5份有轻度局灶性间质及腺周炎症，2份有轻度局灶性腺周炎症。16S rDNA阳性率为19.1%（31/162），慢性前列腺炎标本中为51.0%（26/51），非前列腺炎标本中为4.5%（5/111）（χ² = 29.783，P < 0.01）。在慢性前列腺炎标本中，16S rDNA阳性组IL-1β、TNF-α和NGF的表达明显高于16S rDNA阴性组（P < 0.01）。
细菌性炎症可能在慢性前列腺炎的病因学中起重要作用。
