State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
J Agric Food Chem. 2010 Mar 24;58(6):3512-6. doi: 10.1021/jf904205y.
The deposition profile of melamine was studied in eggs obtained from laying hens fed melamine contaminated feed. A total of 180 laying hens were divided into five groups and were fed diets spiked with 0, 5, 25, 50, or 100 mg of melamine per kg of feed. Eggs collected on days 1, 3, 5, 7, 9, 11, 13, and 15 were analyzed by a gas chromatography-mass spectrometry method, which was fully validated for melamine analysis prior to use. For each treatment group, the melamine level in the eggs was similar from day 1 to day 15 (P > 0.05), suggesting that laying hens did not accumulate melamine for later deposition in eggs. The average melamine concentrations in eggs were 0.00 (below limit of detection), 0.16, 0.47, 0.84, and 1.48 mg/kg for the 0, 5, 25, 50, and 100 mg/kg treatment groups, respectively, which demonstrated an apparent dose-response relationship, and a safety threshold of 164 mg/kg melamine in the feed of laying hen was estimated when a maximum tolerance level of 2.5 mg/kg melamine in egg was adopted. These results provide a scientific basis for the risk assessment of melamine in feeds fed to laying hens.
本研究旨在研究产蛋鸡采食受三聚氰胺污染饲料后，其鸡蛋中三聚氰胺的沉积情况。将 180 只产蛋鸡随机分为 5 组，每组分别采食添加 0、5、25、50 或 100 mg/kg 三聚氰胺的日粮。在第 1、3、5、7、9、11、13 和 15 天收集鸡蛋，采用气相色谱-质谱法进行分析。在使用该方法之前，对其进行了三聚氰胺分析的全面验证。对于每个处理组，鸡蛋中三聚氰胺的水平从第 1 天到第 15 天相似（P＞0.05），这表明产蛋鸡没有将三聚氰胺蓄积起来用于后期在鸡蛋中沉积。0、5、25、50 和 100 mg/kg 处理组鸡蛋中三聚氰胺的平均浓度分别为 0.00（低于检测限）、0.16、0.47、0.84 和 1.48 mg/kg，呈明显的剂量-反应关系。当采用鸡蛋中三聚氰胺最大允许限量 2.5 mg/kg 时，估计饲料中三聚氰胺的安全阈值为 164 mg/kg。这些结果为评估产蛋鸡饲料中三聚氰胺的风险提供了科学依据。
