Institute of Health and Biomedical Innovation and School of Life Sciences, Queensland University of Technology, Australia.
Biol Chem. 2010 Apr;391(4):461-6. doi: 10.1515/BC.2010.043.
PSA-RP2 is a variant transcript expressed from the PSA gene that is conserved in gorillas, chimpanzees and humans suggesting a particular relevance for this transcript in these primates. We demonstrated by qRT-PCR that PSA-RP2 is upregulated in prostate cancer compared with benign prostatic hyperplasia tissues. The PSA-RP2 protein was not detected in seminal fluid and was cytoplasmically localised but not secreted from LNCaP or transfected PC3 prostate cells, despite secretion from transfected Cos-7 and HEK293 kidney cell lines. PSA-RP2-transfected PC3 cells showed slightly decreased proliferation and increased migration towards PC3-conditioned medium that could suggest a functional role in prostate cancer.
PSA-RP2 是一种从 PSA 基因表达的变体转录本，在大猩猩、黑猩猩和人类中保守，表明该转录本在这些灵长类动物中具有特殊的相关性。我们通过 qRT-PCR 证明，与良性前列腺增生组织相比，PSA-RP2 在前列腺癌中上调。PSA-RP2 蛋白未在精液中检测到，并且定位于细胞质，但未从 LNCaP 或转染的 PC3 前列腺细胞中分泌，尽管从转染的 Cos-7 和 HEK293 肾细胞系中分泌。转染 PSA-RP2 的 PC3 细胞显示增殖略有减少，向 PC3 条件培养基的迁移增加，这可能表明其在前列腺癌中具有功能作用。
