通过对 ST 段抬高型心肌梗死患者抽吸血栓切除术中获得的动脉粥样硬化血栓斑块进行免疫组织化学染色，检测到妊娠相关血浆蛋白 A（PAPP-A）的活性：一项简要通讯。
The activity of pregnancy-associated plasma protein A (PAPP-A) as expressed by immunohistochemistry in atherothrombotic plaques obtained by aspiration thrombectomy in patients presenting with a ST-elevation myocardial infarction: a brief communication.
机构信息
Institute of Medicine, University of Bergen, 5021 Bergen, Norway.
出版信息
Thromb J. 2010 Jan 27;8(1):1. doi: 10.1186/1477-9560-8-1.
BACKGROUND
The expression of pregnancy-associated plasma protein A (PAPP-A) was identified by immunohistochemistry (IHC) in culprit atherothrombotic plaque specimens harvested from patients admitted with ST-segment elevation myocardial infarction (STEMI).
METHODS
The atherothrombotic samples were collected from a consecutive cohort consisting of 20 individuals admitted with STEMI to Stavanger University Hospital, Norway, from 2005-2006, presenting angiographically with an acute thrombotic occlusion of a coronary artery characterized by TIMI flow 0. The atherothrombotic plaques were obtained by aspiration thrombectomy during percutaneous coronary intervention within 12 hours from the onset of symptoms and prepared for IHC analysis.
RESULTS
In the IHC analysis staining for PAPP-A occurred in the extracellular matrix of the plaques and no evidence of staining for PAPP-A was found in the thrombi.
CONCLUSION
Our results indicate that in vivo PAPP-A is strongly expressed in atherothrombotic plaques harvested from patients admitted with STEMI, as documented by IHC.
TRIAL REGISTRATION
背景
通过免疫组织化学（IHC）在因 ST 段抬高型心肌梗死（STEMI）入院的患者中采集的致栓性动脉粥样硬化斑块标本中鉴定出妊娠相关血浆蛋白 A（PAPP-A）的表达。
方法
2005-2006 年，连续入选了 20 名因 STEMI 入住挪威斯塔万格大学医院的患者，其通过经皮冠状动脉介入治疗（PCI）采用抽吸血栓切除术，在症状发作后 12 小时内获得了致栓性动脉粥样硬化斑块，这些斑块取自急性血栓闭塞性冠状动脉病变的患者，TIMI 血流 0。对致栓性动脉粥样硬化斑块进行免疫组化分析。
结果
在 IHC 分析中，PAPP-A 在斑块的细胞外基质中发生染色，而在血栓中未发现 PAPP-A 的染色证据。
结论
我们的研究结果表明，在因 STEMI 入院的患者中采集的致栓性动脉粥样硬化斑块中，PAPP-A 在体内强烈表达，这一点通过 IHC 得到了证实。
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