Leturque A, Postic C, Ferre P, Girard J
Centre de Recherche sur la Nutrition, Centre National de la Recherche Scientifique, Meudon-Bellevue, France.
Am J Physiol. 1991 Apr;260(4 Pt 1):E588-93. doi: 10.1152/ajpendo.1991.260.4.E588.
The role of glucose transporters GLUT-1 and GLUT-4 in the development of insulin sensitivity at weaning in rat skeletal muscles and adipose tissue was studied in relation to the nutritional changes when suckling rats shift from a high-fat (HF) to a high-carbohydrate (HCHO) diet. Insulin stimulated the translocation of GLUT-4 protein from an intracellular pool to the plasma membrane in adipocytes from suckling and HCHO- or HF-weaned rats. The GLUT-4 protein and the insulin stimulation were threefold higher in adipocytes from HCHO-weaned rats than in suckling or HF-weaned rats. GLUT-4 mRNA and protein were low in adipose tissue and skeletal muscles of suckling rats and increased two- to threefold in HCHO-weaned rats. This increase was prevented in HF-weaned rats. GLUT-1 mRNA was not affected in both tissues by the developmental stage or the nutritional environment. After feeding HCHO to a suckling rat, GLUT-4 mRNA was threefold increased in 6 days and reached a peak after 4 days in both tissues. The insulin sensitivity of glucose transport in rats at weaning might be conferred by an enhanced expression of GLUT-4, which can be induced within a few hours after feeding a HCHO diet.
研究了葡萄糖转运蛋白GLUT-1和GLUT-4在大鼠骨骼肌和脂肪组织断奶时胰岛素敏感性发育中的作用，该作用与哺乳大鼠从高脂（HF）饮食转变为高碳水化合物（HCHO）饮食时的营养变化相关。胰岛素刺激了来自哺乳大鼠以及HCHO断奶或HF断奶大鼠脂肪细胞中GLUT-4蛋白从细胞内池向质膜的转运。HCHO断奶大鼠脂肪细胞中的GLUT-4蛋白和胰岛素刺激作用比哺乳或HF断奶大鼠高三倍。哺乳大鼠的脂肪组织和骨骼肌中GLUT-4 mRNA和蛋白水平较低，而在HCHO断奶大鼠中增加了两到三倍。这种增加在HF断奶大鼠中受到抑制。GLUT-1 mRNA在这两种组织中均不受发育阶段或营养环境的影响。给哺乳大鼠喂食HCHO后，两种组织中GLUT-4 mRNA在6天内增加了三倍，并在4天后达到峰值。断奶大鼠葡萄糖转运的胰岛素敏感性可能由GLUT-4表达增强所致，喂食HCHO饮食后数小时即可诱导GLUT-4表达增强。
