大鼠骨骼肌中胰岛素敏感性的发展。葡萄糖转运蛋白和胰岛素受体mRNA水平的研究。

Development of insulin sensitivity in rat skeletal muscle. Studies of glucose transporter and insulin receptor mRNA levels.

作者信息

Wallace S, Campbell G, Knott R, Gould G W, Hesketh J

机构信息

Rowett Research Institute, Bucksburn, Aberdeen, UK.

出版信息

FEBS Lett. 1992 Apr 13;301(1):69-72. doi: 10.1016/0014-5793(92)80212-y.

DOI:10.1016/0014-5793(92)80212-y

PMID:1451789

Abstract

Expression of GLUT-4 and insulin receptor mRNAs was investigated in rat skeletal muscle by Northern hybridization. GLUT-4 mRNA was barely detectable in foetal muscle, was expressed at low levels by 1-8 days and at 2-3-fold higher levels during and after weaning (18-40 days). In contrast there was little change in insulin receptor mRNA levels prior to weaning and a reduction in mRNA abundance between 18 and 40 days. Weaning rats on to a diet rich in fat prevented the increase in GLUT-4 abundance seen between 15 and 29 days in animals weaned on a high-carbohydrate diet.

摘要

通过Northern杂交法研究了大鼠骨骼肌中葡萄糖转运蛋白4（GLUT-4）和胰岛素受体mRNA的表达情况。在胎儿肌肉中几乎检测不到GLUT-4 mRNA，在出生后1 - 8天表达水平较低，在断奶期间及断奶后（18 - 40天）表达水平升高2 - 3倍。相比之下，断奶前胰岛素受体mRNA水平变化不大，而在18至40天期间mRNA丰度降低。将断奶大鼠改为富含脂肪的饮食，可阻止在高碳水化合物饮食断奶的动物中15至29天期间出现的GLUT-4丰度增加。