Department of Orthopaedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
Rheumatology (Oxford). 2010 May;49(5):898-906. doi: 10.1093/rheumatology/keq028. Epub 2010 Feb 24.
To determine whether aquaporins (AQPs) are expressed in the synovial tissues of patients with OA and RA, and to examine the patterns of expression in patients with and without hydrarthrosis.
AQPs were detected in synovial tissue samples from patients with OA and RA using RT-PCR and immunohistochemistry. Fibroblast-like synoviocytes (FLSs) from patients with OA and RA were cultured and stimulated with TNF-alpha. The expression of AQPs in FLSs was examined using RT-PCR and western blot analyses and the function of aquaglyceroporins was examined by a glycerol uptake assay.
AQP1, -3 and -9 mRNAs were expressed in synovial tissues from patients with OA and RA. AQP1, -3 and -9 proteins were also detected by immunohistochemistry. AQP9 mRNA was expressed more strongly in the synovial tissues of OA patients with hydrarthrosis than those without. AQP9 mRNA and protein expression were strongly induced with TNF-alpha treatment in FLSs, whereas the expression of AQP1 and -3 mRNAs was not induced with TNF-alpha treatment. AQP9 as an aquaglyceroporin was induced by TNF-alpha.
AQP9 mRNA was detected in synovial tissues from OA and RA patients with hydrarthrosis. AQP9 expression was strongly induced in FLSs with TNF-alpha. Although the functions of AQP1, -3 and -9 in synovial tissues remain to be elucidated, it suggested that AQP9 might be related to the pathogenesis of hydrarthrosis and inflammatory synovitis.
确定水通道蛋白（AQP）是否在 OA 和 RA 患者的滑膜组织中表达，并研究在有无滑液积聚的患者中表达的模式。
使用 RT-PCR 和免疫组织化学检测 OA 和 RA 患者滑膜组织样本中的 AQP。培养 OA 和 RA 患者的成纤维样滑膜细胞（FLS），并用 TNF-α刺激。使用 RT-PCR 和 western blot 分析检测 FLS 中 AQP 的表达，并通过甘油摄取测定检测 aquaglyceroporins 的功能。
AQP1、-3 和 -9 mRNA 在 OA 和 RA 患者的滑膜组织中表达。AQP1、-3 和 -9 蛋白也通过免疫组织化学检测到。OA 患者伴滑液积聚的滑膜组织中 AQP9 mRNA 表达更强。AQP9 mRNA 和蛋白表达在 FLS 中经 TNF-α处理强烈诱导，而 AQP1 和 -3 mRNA 的表达不受 TNF-α处理诱导。AQP9 作为 aquaglyceroporin 被 TNF-α诱导。
AQP9 mRNA 在 OA 和 RA 伴滑液积聚患者的滑膜组织中检测到。AQP9 在 FLS 中经 TNF-α处理强烈诱导。虽然 AQP1、-3 和 -9 在滑膜组织中的功能仍有待阐明，但提示 AQP9 可能与滑液积聚和炎症性滑膜炎的发病机制有关。
