Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Poult Sci. 2010 Mar;89(3):393-400. doi: 10.3382/ps.2009-00281.
The effect of transport stress on superoxide production and adenosine phosphate concentration in addition to avian uncoupling protein (avUCP), avian adenine nucleotide translocator, and avian peroxisome proliferator-activated receptor-gamma coactivator-1alpha mRNA levels of skeletal muscles in broilers was investigated. Arbor Acres chicks (n = 360, 46 d old, males) were randomly allotted to 1 of 5 treatments: unstressed control, 45-min (short-term) transport with 45-min (short-term) recovery, 45-min transport with 3-h (long-term) recovery, 3-h (long-term) transport with 45-min recovery, and 3-h transport with 3-h recovery. Each treatment consisted of 6 replicates with 12 birds each. All birds (except control group) were transported according to a designed protocol. Transport time affected reactive oxygen species production in the thigh muscle (P < 0.05), adenosine triphosphate (ATP) content and energy charge (EC) in both breast and thigh muscles (P < 0.05 for all 4 comparisons), ATP:adenosine diphosphate (ADP) ratio in the breast muscle (P < 0.05), and avUCP mRNA levels in the thigh muscle (P < 0.05). Long-term transport increased (P < 0.05) reactive oxygen species production, ATP content, ATP:ADP ratio, and EC in the thigh muscle, but it decreased ATP content, ATP:ADP ratio, and EC in the breast muscle. Long-term transport increased avUCP mRNA in the thigh muscle (P < 0.05). Long-term recovery increased the ATP (P < 0.05) and ADP (P < 0.05) concentrations, avian adenine nucleotide translocator mRNA (P < 0.05), and avian peroxisome proliferator-activated receptor-gamma coactivator-1alpha mRNA (P < 0.05) in the thigh muscle, whereas EC decreased (P < 0.05) in the breast muscle. There were interactions between transport and recovery time on ATP (P < 0.05), EC (P < 0.05), and avUCP mRNA level (P < 0.05) in the thigh muscle. This study suggests that long-term transport accelerates muscle energy metabolism and lipid peroxidation. A long-term recovery may help alleviate cellular damage and maintain meat quality by reducing the rate of energy metabolism and scavenging of free radicals formed.
研究了运输应激对肉鸡骨骼肌中超氧化物产生和腺苷磷酸盐浓度以及鸟类解偶联蛋白（avUCP）、鸟类腺嘌呤核苷酸转位酶和鸟类过氧化物酶体增殖物激活受体-γ共激活因子-1α mRNA 水平的影响。阿伯罗克斯雏鸡（n = 360，46 日龄，雄性）随机分为 5 种处理之一：未应激对照、45 分钟（短期）运输加 45 分钟（短期）恢复、45 分钟运输加 3 小时（长期）恢复、3 小时（长期）运输加 45 分钟恢复和 3 小时运输加 3 小时恢复。每个处理由 6 个重复组成，每个重复有 12 只鸟。除对照组外，所有鸟类均按设计方案进行运输。运输时间影响大腿肌肉中的活性氧物质产生（P < 0.05）、胸肌和大腿肌肉中的三磷酸腺苷（ATP）含量和能量电荷（EC）（所有 4 个比较均为 P < 0.05）、胸肌中的 ATP：二磷酸腺苷（ADP）比值（P < 0.05）和大腿肌肉中的 avUCP mRNA 水平（P < 0.05）。长期运输增加（P < 0.05）大腿肌肉中的活性氧物质产生、ATP 含量、ATP：ADP 比值和 EC，但降低了胸肌中的 ATP 含量、ATP：ADP 比值和 EC。长期运输增加了大腿肌肉中的 avUCP mRNA（P < 0.05）。长期恢复增加了胸肌中的 ATP（P < 0.05）和 ADP（P < 0.05）浓度、鸟类腺嘌呤核苷酸转位酶 mRNA（P < 0.05）和鸟类过氧化物酶体增殖物激活受体-γ共激活因子-1α mRNA（P < 0.05），而胸肌中的 EC 降低（P < 0.05）。运输和恢复时间之间存在相互作用，影响大腿肌肉中的 ATP（P < 0.05）、EC（P < 0.05）和 avUCP mRNA 水平（P < 0.05）。本研究表明，长期运输加速了肌肉能量代谢和脂质过氧化。长期恢复可以通过降低能量代谢速率和清除自由基来帮助减轻细胞损伤并维持肉品质。
