Department of Pediatrics, Graduate School of Medicine, Kyoto University 54 Kawahara-cho, Shogoin, Sakyo-ku Kyoto 606-8507, Japan.
FASEB J. 2010 Jul;24(7):2245-53. doi: 10.1096/fj.09-137174. Epub 2010 Feb 24.
Induced pluripotent stem (iPS) cells, which are a type of pluripotent stem cell generated from reprogrammed somatic cells, are expected to have potential for patient-oriented disease investigation, drug screening, toxicity tests, and transplantation therapies. Here, we demonstrated that murine iPS cells have the potential to develop in vitro into skeletal muscle stem/progenitor cells, which are almost equivalent to murine embryonic stem cells. Cells with strong in vitro myogenic potential effectively were enriched by fluorescence-activated cell sorting using the anti-satellite cell antibody SM/C-2.6. Furthermore, on transplantation into mdx mice, SM/C-2.6(+) cells exerted sustained myogenic lineage differentiation in injured muscles, while providing long-lived muscle stem cell support. Our data suggest that iPS cells have the potential to be used in clinical treatment of muscular dystrophies.
诱导多能干细胞（iPS 细胞）是一种由重编程体细胞产生的多能干细胞，有望用于面向患者的疾病研究、药物筛选、毒性测试和移植治疗。在这里，我们证明了小鼠 iPS 细胞具有在体外发育为骨骼肌干细胞/祖细胞的潜力，与小鼠胚胎干细胞几乎相当。使用抗卫星细胞抗体 SM/C-2.6 通过荧光激活细胞分选有效地富集具有强体外成肌潜能的细胞。此外，在移植到 mdx 小鼠中后，SM/C-2.6(+)细胞在受损肌肉中持续进行肌源性谱系分化，同时提供长期的肌肉干细胞支持。我们的数据表明，iPS 细胞有可能用于肌肉萎缩症的临床治疗。
