Tamama Kenichi, Kawasaki Haruhisa, Wells Alan
Department of Pathology, The Ohio State University, Columbus, OH 43210, USA.
J Biomed Biotechnol. 2010;2010:795385. doi: 10.1155/2010/795385. Epub 2010 Feb 17.
Adult bone marrow multipotential stromal cells (MSCs) hold great promise in regenerative medicine and tissue engineering. However, due to their low numbers upon harvesting, MSCs need to be expanded in vitro without biasing future differentiation for optimal utility. In this concept paper, we focus on the potential use of epidermal growth factor (EGF), prototypal growth factor for enhancing the harvesting and/or differentiation of MSCs. Soluble EGF was shown to augment MSC proliferation while preserving early progenitors within MSC population, and thus did not induce differentiation. However, tethered form of EGF was shown to promote osteogenic differentiation. Soluble EGF was also shown to increase paracrine secretions including VEGF and HGF from MSC. Thus, soluble EGF can be used not only to expand MSC in vitro, but also to enhance paracrine secretion through drug-releasing MSC-encapsulated scaffolds in vivo. Tethered EGF can also be utilized to direct MSC towards osteogenic lineage both in vitro and in vivo.
成人骨髓多能间充质基质细胞（MSC）在再生医学和组织工程领域极具应用前景。然而，由于收获时其数量较少，MSC需要在体外进行扩增，同时又不能影响其未来的分化能力，以实现最佳应用效果。在这篇概念论文中，我们重点探讨了表皮生长因子（EGF）这种典型生长因子在增强MSC收获和/或分化方面的潜在用途。可溶性EGF已被证明可促进MSC增殖，同时保留MSC群体中的早期祖细胞，因此不会诱导分化。然而，EGF的固定形式已被证明可促进成骨分化。可溶性EGF还被证明可增加MSC的旁分泌分泌，包括VEGF和HGF。因此，可溶性EGF不仅可用于在体外扩增MSC，还可通过体内包裹有药物释放的MSC的支架来增强旁分泌分泌。固定化EGF也可用于在体外和体内引导MSC向成骨谱系分化。
