Kosheverova Vera, Kharchenko Marianna, Kamentseva Rimma, Kotov Michael, Schwarz Alexander, Kuneev Ivan, Kotova Anastasia, Enukashvily Natella, Kornilova Elena
Laboratory of Intracellular Membranes Dynamics, Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky Ave. 4, Saint-Petersburg 194064, Russia.
Institute of Biomedical Systems and Biotechnology, Peter the Great Saint-Petersburg Polytechnic University, Polytechnicheskaya, 29, Saint-Petersburg 195251, Russia.
Int J Mol Sci. 2025 Jul 25;26(15):7201. doi: 10.3390/ijms26157201.
The c-ErbB receptor family is a fundamental cell signaling system that regulates cell proliferation, motility, apoptosis, differentiation, and other key cellular functions. Overexpressed and mutated in some tumors, c-ErbB receptors play a pivotal role in their progression but are also present in many non-malignant cells, including those that are promising from the point of view of regenerative medicine, such as mesenchymal stromal cells (MSCs). The role of c-ErbB receptors in these cells is not clearly understood, and the data on their expression are sporadic. Therefore, the systemic characterization of c-ErbB receptor family expression in MSCs from a wide range of tissues is of high priority. Here, using RT-qPCR and Western blotting analysis, we evaluated the c-ErbB receptors expression pattern at the mRNA and protein levels in human MSCs isolated from six different tissues. We found that MSCs possess considerable and mRNA levels comparable to those in some malignant cells while showing trace and expression. However, EGFR but not HER2 was detected in MSCs at the protein level. We also show that the absence of HER2 protein is not associated with its rapid lysosomal degradation. We conclude that c-ErbB signaling in human MSCs is exclusively mediated by EGFR.
c-ErbB受体家族是一个基本的细胞信号系统，可调节细胞增殖、运动、凋亡、分化及其他关键细胞功能。c-ErbB受体在某些肿瘤中过度表达和发生突变，在肿瘤进展中起关键作用，但也存在于许多非恶性细胞中，包括从再生医学角度来看很有前景的细胞，如间充质基质细胞（MSC）。c-ErbB受体在这些细胞中的作用尚不清楚，其表达数据也很零散。因此，全面表征来自多种组织的MSC中c-ErbB受体家族的表达具有高度优先性。在此，我们使用逆转录定量聚合酶链反应（RT-qPCR）和蛋白质印迹分析，评估了从六种不同组织分离的人MSC中c-ErbB受体在mRNA和蛋白质水平的表达模式。我们发现，MSC具有相当高的 和mRNA水平，与某些恶性细胞中的水平相当，而 和 的表达则很微量。然而，在蛋白质水平上，在MSC中检测到了表皮生长因子受体（EGFR），但未检测到人类表皮生长因子受体2（HER2）。我们还表明，HER2蛋白的缺失与其快速溶酶体降解无关。我们得出结论，人MSC中的c-ErbB信号传导仅由EGFR介导。
