一种新型的负铁缺乏响应元件和一种类似TGGCA型的铁响应元件控制莱茵衣藻中FTR1的表达。
A novel negative Fe-deficiency-responsive element and a TGGCA-type-like FeRE control the expression of FTR1 in Chlamydomonas reinhardtii.
作者信息
Fei Xiaowen, Eriksson Mats, Li Yajun, Deng Xiaodong
机构信息
Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China.
出版信息
J Biomed Biotechnol. 2010;2010:790247. doi: 10.1155/2010/790247. Epub 2010 Feb 22.
We have reported three Fe-deficiency-responsive elements (FEREs), FOX1, ATX1, and FEA1, all of which are positive regulatory elements in response to iron deficiency in Chlamydomonas reinhardtii. Here we describe FTR1, another iron regulated gene and mutational analysis of its promoter. Our results reveal that the FeREs of FTR1 distinguish itself from other iron response elements by containing both negative and positive regulatory regions. In FTR1, the -291/-236 region from the transcriptional start site is necessary and sufficient for Fe-deficiency-inducible expression. This region contains two positive FeREs with a TGGCA-like core sequence: the FtrFeRE1 (ATGCAGGCT) at -287/-279 and the FtrFeRE2 (AAGCGATTGCCAGAGCGC) at -253/-236. Furthermore, we identified a novel FERE, FtrFeRE3 (AGTAACTGTTAAGCC) localized at -319/-292, which negatively influences the expression of FTR1.
我们已经报道了三种缺铁应答元件（FEREs），即FOX1、ATX1和FEA1，它们在莱茵衣藻对缺铁的应答中均为正向调控元件。在此，我们描述了另一个铁调控基因FTR1及其启动子的突变分析。我们的结果表明，FTR1的铁应答元件（FeREs）通过同时包含负向和正向调控区域而有别于其他铁应答元件。在FTR1中，转录起始位点上游-291/-236区域对于缺铁诱导表达是必需且充分的。该区域包含两个具有TGGCA样核心序列的正向FeREs：位于-287/-279的FtrFeRE1（ATGCAGGCT）和位于-253/-236的FtrFeRE2（AAGCGATTGCCAGAGCGC）。此外，我们鉴定出一个新的FEREs，即位于-319/-292的FtrFeRE3（AGTAACTGTTAAGCC），它对FTR1的表达有负向影响。
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