Biomedical Engineering Research Centre, Nanyang Technological University, Singapore.
J Microbiol Immunol Infect. 2009 Oct;42(5):364-70.
Antibiotic combinations are used to enhance antibacterial efficacy and to prevent the development of resistance. In this study, the in vitro activities of antibiotic and phytochemical combinations against Pseudomonas aeruginosa were tested by the fractional inhibitory concentration method, derived from the minimal inhibitory concentrations (MICs) of the agents in combination.
The antimicrobial activity of phytochemicals, alone and in combination with antibiotics, was evaluated using the checkerboard assay and time-kill curve methods.
There was synergism between gentamicin and caffeic acid, and sulfadiazine and the 3 phytochemicals under investigation (protocatechuic acid, quercetin, caffeic acid). The MIC of sulfadiazine was 256 microg/mL, and of gentamicin was 2 microg/mL. When gentamicin was combined with one-quarter the MIC of caffeic acid, the MIC of gentamicin was reduced 4-fold. When sulfadiazine was tested with one-quarter the MIC of protocatechuic acid, quercetin, and caffeic acid, the MIC was reduced 4-fold in combination with each of the drugs.
These results indicate the potential efficacy of phytochemicals in combination with antibiotics for enhancing total biological activity.
抗生素联合使用可增强抗菌效果，防止耐药性的产生。在本研究中，采用微量稀释法（基于药物单独 MIC 值），通过棋盘微量稀释法对植物化学物质与铜绿假单胞菌的组合的体外活性进行了测试。
采用棋盘微量稀释法和时间杀伤曲线法评估植物化学物质单独及与抗生素联合的抗菌活性。
氨基糖苷类抗生素庆大霉素与咖啡酸，磺胺嘧啶与我们研究的 3 种植物化学物质（原儿茶酸、槲皮素、咖啡酸）之间存在协同作用。磺胺嘧啶的 MIC 值为 256 μg/mL，庆大霉素的 MIC 值为 2 μg/mL。当庆大霉素与咖啡酸的四分之一 MIC 联合使用时，庆大霉素的 MIC 值降低了 4 倍。当磺胺嘧啶与原儿茶酸、槲皮素和咖啡酸的四分之一 MIC 联合使用时，与每种药物联合使用时，磺胺嘧啶的 MIC 值均降低了 4 倍。
这些结果表明，植物化学物质与抗生素联合使用具有增强总生物活性的潜力。
