Component Composition and Antimicrobial Activity of CO Extract of , Growing in the Territory of Kazakhstan.

In the medicine of many countries, the use of herbal healing agents included a significant contribution to improving human health and well-being. Many antibiotics have been widely used to treat infectious diseases caused by various pathogenic bacteria. However, increased multidrug resistance has led to increased severity of diseases caused by bacterial pathogens. Bacteria remain the main causative agents of diseases that cause human death, even in the present day. This cause prompted scientists to investigate alternative new molecules against bacterial strains. The significant interest for the study is L. (family ), a widespread annual plant used in folk medicine. Thus, the production and study of CO extract of is an actual problem. . Raw materials were collected from Almaty and Zhambyl regions (Southeast and South Kazakhstan) in phase flowering. herb's CO extract was obtained by subcritical carbon dioxide extraction (installation of carbon dioxide flow-through extraction- 5L). The Wiley 7 edition and NIST'02 library were used to identify the mass spectra obtained. The antimicrobial activity study was conducted by the micromethod of serial dilution and disco-diffuse method. Standard test strains of microorganisms were used: ATCC 6633, ATCC 6538-P, ATCC 10231, and ATCC 8739. . The use of carbon dioxide extraction (further CO extract) is a promising direction of obtaining total medicinal substances containing biologically active substances, from fractions of volatile esters of various composition and functional purpose until a fraction of fatty acids and fat-soluble vitamins. In the current study, we obtained CO extract at subcritical conditions from aboveground organs of and investigated the component composition for the first time. From 41 to 66 components were identified in the composition of 's CO extract. Studies of antimicrobial activity showed that CO extract of had the expressed effect against clinically significant microorganisms such as , , , and . . This study showed that CO extract of 's raw material contained biological active compounds exhibiting a significant antimicrobial effect.