Key Laboratory of Biofuel, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266071, China.
Appl Microbiol Biotechnol. 2010 Jun;87(1):117-26. doi: 10.1007/s00253-010-2484-8. Epub 2010 Feb 25.
Ionic liquid (IL) pretreatment of lignocellulose materials is a promising process in biomass conversion to renewable biofuel. More in-depth research involving environment-friendly IL is much needed to explore pretreatment green route. In our case, IL 1-methyl-3-methylimidazolium dimethylphosphite ([Mmim]DMP) was chosen as an environment-friendly solvent to pretreat corn cob in view of its biocompatibility with both lignocellulose solubility and cellulase activity. The pretreatment/saccharification process and in situ saccharification process involving [Mmim]DMP were efficiently performed in bioconversion of corn cob to sugars, and more than 70% saccharification rates were obtained. Furthermore, the fermentability of reducing sugars obtained from the hydrolyzates was evaluated using Rhodococcus opacus strain ACCC41043 (R. opacus). High lipid production 41-43% of cell dry matter was obtained after 30 h of cultivation. GC/MS analysis indicated that lipids from R. opacus contained mainly long-chain fatty acids with four major constituent/oleic acid, stearic acid, palmitic acid, palmitoleic acid which are good candidates for biodiesel. These elucidated that corn cob pretreated by IL [Mmim]DMP did not bring negative effects on saccharification, cell growth, and accumulation of lipid of R. opacus. In conclusion, the IL [Mmim]DMP shows promise as green pretreatment solvent for cellulosic materials.
离子液体（IL）预处理木质纤维素材料是生物质转化为可再生生物燃料的有前途的过程。需要更深入地研究涉及环境友好型 IL 的预处理绿色途径。在我们的案例中，考虑到离子液体 1-甲基-3-甲基咪唑二甲基磷酸酯([Mmim]DMP)与木质纤维素的溶解度和纤维素酶活性的生物相容性，选择[Mmim]DMP 作为环境友好型溶剂来预处理玉米芯。预处理/糖化过程和涉及[Mmim]DMP 的原位糖化过程在玉米芯转化为糖的生物转化中得到了有效进行，糖化率超过 70%。此外，使用 Rhodococcus opacus 菌株 ACCC41043（R.opacus）评估了从水解物中获得的还原糖的发酵性。在培养 30 小时后，获得了 41-43%细胞干物质的高油脂产量。GC/MS 分析表明，R.opacus 的油脂主要含有长链脂肪酸，其中四种主要成分/油酸、硬脂酸、棕榈酸、棕榈油酸是生物柴油的良好候选物。这些结果表明，IL [Mmim]DMP 预处理的玉米芯不会对 R.opacus 的糖化、细胞生长和油脂积累产生负面影响。总之，IL [Mmim]DMP 作为纤维素材料的绿色预处理溶剂具有很大的潜力。
