AVT-Enzyme Process Technology, RWTH Aachen University, Aachen, Germany.
Bioresour Technol. 2012 Jul;115:27-34. doi: 10.1016/j.biortech.2011.10.080. Epub 2011 Oct 30.
For the efficient production of glucose for platform chemicals or biofuels, cellulosic biomass is pretreated and subsequently hydrolyzed with cellulases. Although ionic liquids (IL) are known to effectively pretreat cellulosic biomass, the hydrolysis of IL pretreated biomass has not been optimized so far. Here, we present a semi-empirical model to rationally optimize the hydrolysis of pretreated α-cellulose - regenerated from IL and containing residual IL from the pretreatment. First, the influence of the IL MMIM DMP on the individual cellulases endoglucanase I, cellobiohydrolase I and β-glucosidase was investigated. Second, an enzyme loading-dependent model was developed to describe kinetics for the individual cellulases and cellulase mixtures. Third, this model was used to optimize the cellulase mixture for the efficient hydrolysis of regenerated cellulose containing residual IL. Finally, we could significantly increase the initial hydrolysis rate in 10% (v/v) MMIM DMP by 49% and the sugar yield by 10% points.
为了高效生产用于平台化学品或生物燃料的葡萄糖，纤维素生物质首先经过预处理，然后用纤维素酶进行水解。尽管离子液体（IL）已被证实可有效地预处理纤维素生物质，但 IL 预处理生物质的水解尚未得到优化。在此，我们提出了一种半经验模型，以合理优化从 IL 中再生的预处理 α-纤维素（其中含有预处理过程中残留的 IL）的水解过程。首先，我们考察了 IL MMIM DMP 对内切葡聚糖酶 I、纤维二糖水解酶 I 和 β-葡萄糖苷酶这三种单一纤维素酶的影响。其次，我们开发了一种酶加载依赖性模型来描述这三种单一纤维素酶和纤维素酶混合物的动力学。然后，我们使用该模型对含有残留 IL 的再生纤维素的纤维素酶混合物进行了优化，以实现高效水解。最终，我们可以使在 10%（v/v）MMIM DMP 中再生纤维素的初始水解速率显著提高 49%，并使糖产率提高 10 个百分点。
