Shahid Chamran University, Ahvaz, Iran.
J Med Toxicol. 2010 Mar;6(1):22-6. doi: 10.1007/s13181-010-0030-9.
In pathologic conditions or poisoning states, iron overload can affect different tissues including liver. In this study, the prophylactic effect of deferoxamine and silymarin was compared in decreasing experimental iron-overload-induced hepatotoxicity in rats. The study was done in six groups of rats, which received drugs q2 days for 2 weeks. The rats in groups 1 to 6 received drugs, respectively: normal saline, iron dextran, iron dextran + deferoxamine (intraperitoneally), iron dextran + silymarin (orally), iron dextran + silymarin (intraperitoneally), and iron dextran + deferoxamine (intraperitoneally) + silymarin (intraperitoneally). At the end of the study, blood was collected, and serum was separated for laboratory tests. The liver of rats was separated for iron measuring and tissue processing. The serum iron concentration and the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity were determined. The numbers of necrotic hepatocytes were counted as quantity index tissue injury in light microscopic examination. The mean of serum and liver iron in group 2 was significantly greater than group 1. Liver iron was significantly decreased in other groups except group 4. Also serum iron was decreased in groups 3 to 6 compared to group 2 (nearly 400%). ALT activity in group 3 and AST activity in group 5 were significantly lesser than in other groups. The mean of necrotic hepatocytes in group 2 was significantly increased in comparison to group 1. This elevation was significantly prevented by deferoxamine and silymarin. The result of the present study shows that silymarin has a protective effect similar to deferoxamine on iron overload-induced hepatotoxicity.
在病理条件或中毒状态下,铁过载会影响包括肝脏在内的不同组织。在这项研究中,比较了去铁胺和水飞蓟素在减少大鼠实验性铁过载诱导的肝毒性中的预防作用。该研究在六组大鼠中进行,这些大鼠每两天接受一次药物治疗,共两周。第 1 至 6 组大鼠分别接受以下药物治疗:生理盐水、右旋糖酐铁、右旋糖酐铁+去铁胺(腹腔内)、右旋糖酐铁+水飞蓟素(口服)、右旋糖酐铁+水飞蓟素(腹腔内)和右旋糖酐铁+去铁胺(腹腔内)+水飞蓟素(腹腔内)。研究结束时,采集血液并分离血清进行实验室检查。分离大鼠肝脏进行铁测量和组织处理。测定血清铁浓度和血清丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)活性。在光镜检查中,计数坏死肝细胞的数量作为组织损伤的定量指标。第 2 组的血清和肝脏铁含量明显高于第 1 组。除第 4 组外,其他组的肝脏铁含量均有所降低。与第 2 组相比,第 3 组至第 6 组的血清铁含量也有所降低(近 400%)。第 3 组的 ALT 活性和第 5 组的 AST 活性明显低于其他组。与第 1 组相比,第 2 组的坏死肝细胞数量明显增加。这种增加被去铁胺和水飞蓟素显著预防。本研究结果表明,水飞蓟素对铁过载诱导的肝毒性具有与去铁胺相似的保护作用。