Institute of Meat Hygiene and Technology, Belgrade, Serbia.
J Environ Sci Health B. 2009 Nov;44(8):781-7. doi: 10.1080/03601230903238327.
This study was undertaken to compare two different analytical methods for the determination and confirmation of ochratoxin A (OTA) in blood serum, kidney and liver of pigs. Sample clean-up was based on liquid-liquid phase extraction. The detection of OTA was accomplished with high-performance liquid chromatography (HPLC) combined either with fluorescence detection (FL) or electro spray ionization (ESI+) tandem mass spectrometry (MS-MS). Comparative method evaluation was based on the investigation of 90 samples of blood serum, kidney and liver per animal originating from different regions of Serbia. The analytical results are discussed in view of the respective method validation data and the corresponding experimental protocols. In general, analytical data obtained with (LC-MS-MS) liquid chromatography electro spray tandem mass spectro metry detection offered comparable good results at the sub-ppb concentration level. The results indicate that the liquid chromatography electro spray tandem mass spectrometric (LC-MS/MS) method was more specific and sensitive for the analysis and confirmation of ochratoxin A in pig tissues then high pressure liquid chromatography (HPLC) method after methylation of OTA.
本研究旨在比较两种不同的分析方法，用于检测和确证猪血清、肾脏和肝脏中的赭曲霉毒素 A（OTA）。样品的净化基于液-液萃取。OTA 的检测采用高效液相色谱（HPLC）结合荧光检测（FL）或电喷雾电离（ESI+）串联质谱（MS-MS）完成。比较方法的评估基于对来自塞尔维亚不同地区的 90 个动物的血清、肾脏和肝脏样本的调查。根据各自的方法验证数据和相应的实验方案讨论了分析结果。总的来说，在亚 ppb 浓度水平下，用（LC-MS-MS）液相色谱电喷雾串联质谱检测获得的分析数据具有相当好的结果。结果表明，与 OTA 甲基化后的高效液相色谱（HPLC）法相比，液质联用（LC-MS/MS）法在分析和确证猪组织中的赭曲霉毒素 A 时更具特异性和灵敏度。
