Köster A, Landgraf S, Leipold A, Sachse R, Gebhart E, Tulusan A H, Rónay G, Schmidt C, Dingermann T
Institut für Biochemie, Medizinischen Fakultät, Universität Erlangen-Nürnberg, F.R.G.
Anticancer Res. 1991 Jan-Feb;11(1):193-201.
More than 60 breast cancer specimens were screened for their expression status of 25 different proto-oncogenes. The screening method is based on in vitro synthesis of a radioactive cDNA copied from the total cellular RNA of tumor tissue. This cDNA is hybridized to cloned oncogene probes which are immobilized to a GeneScreen membrane. Frequently multiple oncogenes were found expressed although expression levels were rather moderate. 25-30% of the analyzed tumors showed significant expression of either erbB, src, raf1, lck or H-ras. Although neu expression--an oncogene believed to be particular relevant as prognostic parameter for mamma carcinoma--was screened for most of the tumors with a heterologous gene probe, expression signals could be detected in about 20% cases. The only notable correlation with classical clinical parameters such as tumor size and proliferation stage, hormone receptor status and different DNA indices was the observation that tumors lacking the progesterone receptor frequently express multiple oncogenes. Advantages and limitations of the cDNA/dot-blot screening for oncogene expression are discussed.
对60多个乳腺癌标本进行了25种不同原癌基因表达状态的筛查。筛查方法基于从肿瘤组织的总细胞RNA体外合成放射性cDNA。该cDNA与固定在GeneScreen膜上的克隆癌基因探针杂交。尽管表达水平相当适中，但经常发现多个癌基因表达。25%-30%的分析肿瘤显示erbB、src、raf1、lck或H-ras中有显著表达。尽管大多数肿瘤用异源基因探针筛查了neu表达（一种被认为与乳腺癌预后参数特别相关的癌基因），但在约20%的病例中可检测到表达信号。与肿瘤大小、增殖阶段、激素受体状态和不同DNA指数等经典临床参数唯一显著的相关性是观察到缺乏孕激素受体的肿瘤经常表达多个癌基因。讨论了cDNA/斑点印迹法筛查癌基因表达的优缺点。
