Wang Weimin, Zhao Jianwei, Han Qianwei, Wang Gang, Yang Gengcheng, Shallop Anthony J, Liu Jane, Gaffney Barbara L, Jones Roger A
Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
Nucleosides Nucleotides Nucleic Acids. 2009 May;28(5):424-34. doi: 10.1080/15257770903044234.
(15)N NMR chemical shift changes in the presence of Mg(H(2)O)(6)(2+), Zn(2+), Cd(2+), and Co(NH(3))(6)(3+) were used to probe the effect of flanking bases on metal binding sites in three different RNA motifs. We found that: for GC pairs, the presence of a flanking purine creates a site for the soft metals Zn(2+) and Cd(2+) only; a GG.UU motif selectively binds only Co(NH(3))(6)(3+), while a UG.GU motif binds none of these metals; a 3' guanosine flanking the adenosine of a sheared GA.AG pair creates an unusually strong binding site that precludes binding to the cross-strand stacked guanosines within the tandem pair.
利用在Mg(H₂O)₆²⁺、Zn²⁺、Cd²⁺和Co(NH₃)₆³⁺存在下的¹⁵N核磁共振化学位移变化,来探究侧翼碱基对三种不同RNA基序中金属结合位点的影响。我们发现:对于GC碱基对,侧翼嘌呤的存在仅为软金属Zn²⁺和Cd²⁺创造了一个位点;GG.UU基序仅选择性结合Co(NH₃)₆³⁺,而UG.GU基序不结合这些金属中的任何一种;剪切后的GA.AG对中腺苷侧翼的3'鸟苷创造了一个异常强的结合位点,该位点阻止了与串联对中跨链堆积鸟苷的结合。