Xiang Zheng, He Fan, Kang Ting-Guo, Dou De-Qiang, Gai Kuo, Shi Yu-Yuan, Kim Young-Ho, Dong Feng
College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
Nat Prod Commun. 2010 Jan;5(1):95-8.
The inhibitory effect of smallanthaditerpenic acids A, B, C and D previously isolated from leaves of Smallanthus sonchifolius (yacon) on alpha-glucosidase were examined and their IC50 were determined to be 0.48 mg/mL, 0.59 mg/mL, 1.00 mg/mL, and 1.17 mg/mL respectively. In addition, a rapid, reliable RP-HPLC method for the analysis of chlorogenic acid, caffeic acid, and smallanthaditerpenic acids A and C in yacon leaves was established, and the variation in their contents in leaves from plants cultivated in different places and collected at different times of the year were compared. The established analytical method for determining smallanthaditerpenic acids A and C, chlorogenic acid and caffeic acid presented good results and could be used as a method for the quality control of S. sonchifolius leaves.
对先前从雪莲果（菊薯）叶片中分离得到的小花菊萜酸A、B、C和D对α-葡萄糖苷酶的抑制作用进行了研究，其半数抑制浓度（IC50）分别测定为0.48毫克/毫升、0.59毫克/毫升、1.00毫克/毫升和1.17毫克/毫升。此外，建立了一种快速、可靠的反相高效液相色谱法（RP-HPLC）用于分析雪莲果叶片中的绿原酸、咖啡酸以及小花菊萜酸A和C，并比较了不同产地和不同采收时间的植株叶片中它们含量的变化。所建立的测定小花菊萜酸A和C、绿原酸和咖啡酸的分析方法结果良好，可作为雪莲果叶片质量控制的方法。
