含次黄嘌呤（肌苷）的 DNA 双链的稳定性：气相与溶液相及生物学意义。

Stability of DNA duplexes containing hypoxanthine (inosine): gas versus solution phase and biological implications.

机构信息

Department of Chemistry and Chemical Biology Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA.

出版信息

J Org Chem. 2010 Mar 19;75(6):1848-54. doi: 10.1021/jo9023683.

DOI:10.1021/jo9023683

PMID:20184296

Abstract

A series of 9-mer DNA duplexes of the sequence 5'-d(GGTTXTTGG)-3'/3'-d(CCAAYAACC)-5', where the central base X or Y = adenine (A), guanine (G), thymine (T), cytosine (C), and hypoxanthine (H), have been examined toward understanding the effect of hypoxanthine on DNA stability. Comparison of the duplex stability in the gas phase versus solution indicates that hypoxanthine has much less of a destabilizing effect in the gas phase versus in solution, relative to the normal complementary duplexes. The biological implications of these results, both in the context of hypoxanthine as a universal base and as a damaged base, are discussed.

摘要

已研究了一系列 9 -mer DNA 双链体，其序列为 5'-d(GGTTXTTGG)-3'/3'-d(CCAAYAACC)-5'，其中中央碱基 X 或 Y = 腺嘌呤（A）、鸟嘌呤（G）、胸腺嘧啶（T）、胞嘧啶（C）和次黄嘌呤（H），旨在了解次黄嘌呤对 DNA 稳定性的影响。与正常互补双链体相比，气相中和溶液中次黄嘌呤的双链体稳定性比较表明，在气相中，次黄嘌呤的破坏稳定性的影响要小得多。讨论了这些结果在次黄嘌呤作为通用碱基和受损碱基方面的生物学意义。