利用一种新型荧光指示剂——钠结合苯并呋喃间苯二甲酸酯对豚鼠心肌细胞内游离钠离子进行定量分析。
Quantification of intracellular free sodium ions by using a new fluorescent indicator, sodium-binding benzofuran isophthalate in guinea pig myocytes.
作者信息
Satoh H, Hayashi H, Noda N, Terada H, Kobayashi A, Yamashita Y, Kawai T, Hirano M, Yamazaki N
机构信息
Third Department of Internal Medicine, Hamamatsu University School of Medicine, Japan.
出版信息
Biochem Biophys Res Commun. 1991 Mar 15;175(2):611-6. doi: 10.1016/0006-291x(91)91609-g.
Isolated guinea pig myocytes were loaded with the Na(+)-sensitive fluorescent probe, sodium-binding benzofuran isophthalate (SBFI). The 340/380 nm fluorescence ratios were measured with fluorescence microscopy. The distribution of intracellular Na+ concentration ([Na+]i) was homogenous, and the mean resting [Na+]i was 8.4 +/- 0.5 mM. There was a significant relationship (r = 0.66, p less than 0.001) between elevation of [Na+]i and shortening of longitudinal length of the cells, during the perfusion of 100 microM strophanthidin. It is concluded that this method is suitable for measuring [Na+]i in isolated myocytes.
将分离的豚鼠心肌细胞用对钠离子敏感的荧光探针——钠结合苯并呋喃异邻苯二甲酸酯（SBFI）进行负载。通过荧光显微镜测量340/380nm荧光比率。细胞内钠离子浓度（[Na⁺]i）分布均匀，静息状态下[Na⁺]i的平均值为8.4±0.5mM。在灌注100μM毒毛花苷期间，[Na⁺]i的升高与细胞纵向长度的缩短之间存在显著相关性（r = 0.66，p<0.001）。得出结论，该方法适用于测量分离心肌细胞中的[Na⁺]i。
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