Satoh H, Hayashi H, Noda N, Terada H, Kobayashi A, Yamashita Y, Kawai T, Hirano M, Yamazaki N
Third Department of Internal Medicine, Hamamatsu University School of Medicine, Japan.
Biochem Biophys Res Commun. 1991 Mar 15;175(2):611-6. doi: 10.1016/0006-291x(91)91609-g.
Isolated guinea pig myocytes were loaded with the Na(+)-sensitive fluorescent probe, sodium-binding benzofuran isophthalate (SBFI). The 340/380 nm fluorescence ratios were measured with fluorescence microscopy. The distribution of intracellular Na+ concentration ([Na+]i) was homogenous, and the mean resting [Na+]i was 8.4 +/- 0.5 mM. There was a significant relationship (r = 0.66, p less than 0.001) between elevation of [Na+]i and shortening of longitudinal length of the cells, during the perfusion of 100 microM strophanthidin. It is concluded that this method is suitable for measuring [Na+]i in isolated myocytes.
将分离的豚鼠心肌细胞用对钠离子敏感的荧光探针——钠结合苯并呋喃异邻苯二甲酸酯（SBFI）进行负载。通过荧光显微镜测量340/380nm荧光比率。细胞内钠离子浓度（[Na⁺]i）分布均匀，静息状态下[Na⁺]i的平均值为8.4±0.5mM。在灌注100μM毒毛花苷期间，[Na⁺]i的升高与细胞纵向长度的缩短之间存在显著相关性（r = 0.66，p<0.001）。得出结论，该方法适用于测量分离心肌细胞中的[Na⁺]i。
