视黄酸调节的肝素结合蛋白RI-HB的分子克隆

Molecular cloning of RI-HB, a heparin binding protein regulated by retinoic acid.

作者信息

Urios P, Duprez D, Le Caer J P, Courtois Y, Vigny M, Laurent M

机构信息

Unité de Recherches Gérontologiques, INSERM U 118 CNRS UA 630, Paris, France.

出版信息

Biochem Biophys Res Commun. 1991 Mar 15;175(2):617-24. doi: 10.1016/0006-291x(91)91610-o.

DOI:10.1016/0006-291x(91)91610-o

PMID:2018506

Abstract

RI-HB is an extracellular heparin binding protein regulated by retinoic acid and essentially expressed during embryogenesis. This study reports the cloning and sequencing of the cDNA that encodes RI-HB. The sequence of RI-HB contains 121 amino acid residues and is very rich in basic amino acids and cysteines. This sequence was compared to those of HBGAM and MK protein, two other heparin binding proteins exhibiting growth and/or neurotrophic activities. Northern blot analysis indicates that RI-HB mRNA is strongly expressed during early chicken embryogenesis and that it is induced by retinoic acid treatment of chicken fibroblasts and myotubes in culture.

摘要

RI-HB是一种细胞外肝素结合蛋白，受视黄酸调节，在胚胎发生过程中基本表达。本研究报道了编码RI-HB的cDNA的克隆和测序。RI-HB的序列包含121个氨基酸残基，富含碱性氨基酸和半胱氨酸。该序列与另外两种具有生长和/或神经营养活性的肝素结合蛋白HBGAM和MK蛋白的序列进行了比较。Northern印迹分析表明，RI-HB mRNA在鸡胚胎发育早期强烈表达，并且在培养的鸡成纤维细胞和肌管中经视黄酸处理后被诱导表达。