Department of Biomedical Engineering, Advanced Medical Engineering Center, National Cardiovascular Center Research Institute, Suita, Japan.
Biomaterials. 2010 May;31(14):4231-7. doi: 10.1016/j.biomaterials.2010.01.126. Epub 2010 Feb 24.
Here, we report that two distinctive cell populations with osteoblastic differentiation ability were found in adherent cell populations from bone marrow. Mesenchymal stem cells (MSCs) were conventionally isolated by using adherent property of bone marrow cells onto a plastic culture dish. MSCs enriched on the basis of their adherent property were considered phenotypically and functionally heterogeneous. We developed a ligand-immobilized surface for separating subpopulation of adherent cells derived from bone marrow by the cell rolling process. We successfully isolate two cell populations with high differentiation ability for osteoblasts in adherent bone marrow cells by using the anti-CD34 antibody-immobilized column. The antibody was covalently conjugated with polyacrylic acid and introduced onto the inner surface of a silicone tube. When cell suspension of MSCs was injected into the antibody-immobilized column, different cell populations were isolated. After the cultivation of isolated cells in the osteoblastic differentiation medium for 1 week, few sub-populations were strongly induced to form osteoblastic cells. This study revealed that the ligand-immobilized surface can be used to continually separate cell populations under a labeling-free condition.
在这里，我们报告在骨髓贴壁细胞群体中发现了具有成骨分化能力的两种不同的细胞群体。间充质干细胞（MSCs）通常通过利用骨髓细胞在塑料培养皿上的贴壁特性进行分离。基于其贴壁特性富集的 MSCs 在表型和功能上表现出异质性。我们开发了一种配体固定表面，通过细胞滚动过程分离骨髓来源的贴壁细胞的亚群。我们成功地通过抗 CD34 抗体固定柱从贴壁骨髓细胞中分离出两种具有高成骨分化能力的细胞群体。该抗体通过共价键与聚丙烯酸结合，并引入硅酮管的内表面。当 MSC 的细胞悬浮液注入抗体固定柱时，不同的细胞群体被分离。在成骨分化培养基中培养分离的细胞 1 周后，少数亚群被强烈诱导形成成骨细胞。这项研究表明，配体固定表面可用于在无标记条件下连续分离细胞群体。
