EA3952, Cellular and Tissular Bioengineering Laboratory, Henri Mondor Hospital, Paris-EST University, France.
Biomaterials. 2010 Jan;31(2):270-8. doi: 10.1016/j.biomaterials.2009.09.043. Epub 2009 Sep 26.
Culture of expanded mesenchymal stem cells (MSCs) seeded on biomaterials may represent a clinical alternative to autologous bone graft in bone regeneration. Foetal bovine serum (FBS) is currently used for MSC expansion, despite risks of infectious disease transmission and immunological reaction due to its xenogenic origin. This study aimed to compare the osteogenic capacities of clinical-grade human MSCs cultured with FBS or allogenic human platelet lysate (PL). In vitro, MSCs cultured in PL both accelerate the expansion rate over serial passages and spontaneously induce osteoblastic gene expression such as alkaline phosphatase (ALP), bone sialoprotein (BSP), osteopontin (Op) and bone morphogenetic protein-2 (BMP-2). In vivo, ectopic bone formation is only observed on ceramics seeded with MSCs grown in PL medium implanted under the skin of immunodeficient mice for 7 weeks. In conclusion, allogenic human PL accelerates MSC proliferation and enhances MSC osteogenic differentiation.
在生物材料上种植扩增的间充质干细胞（MSCs）的培养物可能代表了骨再生中自体骨移植物的临床替代物。目前，胎牛血清（FBS）被用于 MSC 的扩增，尽管由于其异源来源，存在传染病传播和免疫反应的风险。本研究旨在比较使用 FBS 或同种异体人血小板裂解物（PL）培养的临床级人 MSC 的成骨能力。在体外，PL 中培养的 MSC 不仅加速了传代过程中的扩增速度，而且还自发诱导成骨细胞基因表达，如碱性磷酸酶（ALP）、骨涎蛋白（BSP）、骨桥蛋白（Op）和骨形态发生蛋白-2（BMP-2）。在体内，仅在 PL 培养基中培养的 MSC 种植在陶瓷上，在免疫缺陷小鼠的皮肤下植入 7 周后才观察到异位骨形成。总之，同种异体人 PL 可加速 MSC 的增殖并增强 MSC 的成骨分化。
