Department of Microbiology and Carol Yu Centre for Infection, University of Hong Kong, Hong Kong SAR.
Department of Clinical Pathology, Tuen Mun Hospital, Hong Kong SAR.
J Med Microbiol. 2010 Jun;59(Pt 6):702-707. doi: 10.1099/jmm.0.015032-0. Epub 2010 Feb 25.
A bacterial collection (n=249) obtained in Hong Kong from 2002 to 2004 was used to investigate the molecular epidemiology of aminoglycoside resistance among Escherichia coli isolates from humans and food-producing animals. Of these, 89 isolates were gentamicin-sensitive (human n=60, animal n=29) and 160 isolates were gentamicin-resistant (human n=107, animal n=53). Overall, 84.1% (90/107) and 75.5% (40/53) of the gentamicin-resistant isolates from human and animal sources, respectively, were found to possess the aacC2 gene. The aacC2 gene for 20 isolates (10 each for human and animal isolates) was sequenced. Two alleles were found that were equally distributed in human and animal isolates. PFGE showed that the gentamicin-resistant isolates exhibited diverse patterns with little clonality. In some isolates, the aacC2 gene was encoded on large transferable plasmids of multiple incompatibility groups (IncF, IncI1 and IncN). An IncFII plasmid of 140 kb in size was shared by one human and three animal isolates. In summary, this study showed that human and animal isolates share the same pool of resistance genes.
本研究旨在调查香港地区人源和动物源大肠杆菌对氨基糖苷类药物耐药的分子流行病学特征。2002 年至 2004 年，从香港地区收集了 249 株细菌用于研究。其中，89 株为庆大霉素敏感株（人源 60 株，动物源 29 株），160 株为庆大霉素耐药株（人源 107 株，动物源 53 株）。总的来说，人源和动物源庆大霉素耐药株中分别有 84.1%（90/107）和 75.5%（40/53）携带 aacC2 基因。对 20 株（人源和动物源各 10 株）庆大霉素耐药株进行了 aacC2 基因测序。发现两种等位基因在人源和动物源耐药株中分布均匀。PFGE 显示，庆大霉素耐药株表现出多种不同的模式，具有较少的克隆性。在一些耐药株中，aacC2 基因位于多个不相容群（IncF、IncI1 和 IncN）的可移动大质粒上。一个大小为 140kb 的 IncFII 质粒在 1 个人源和 3 个动物源耐药株中共享。综上所述，本研究表明人源和动物源耐药株共享相同的耐药基因库。
