Laboratory of Population Genetics, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America.
PLoS One. 2010 Feb 23;5(2):e9355. doi: 10.1371/journal.pone.0009355.
Recent studies have shown a genetic influence on gene expression variation, chromatin, and DNA methylation. However, the effects of genetic background and tissue types on DNA methylation at the genome-wide level have not been characterized extensively. To study the effect of genetic background and tissue types on global DNA methylation, we performed DNA methylation analysis using the Affymetrix 500K SNP array on tumor, adjacent normal tissue, and blood DNA from 30 patients with esophageal squamous cell carcinoma (ESCC). The use of multiple tissues from 30 individuals allowed us to evaluate variation of DNA methylation states across tissues and individuals. Our results demonstrate that blood and esophageal tissues shared similar DNA methylation patterns within the same individual, suggesting an influence of genetic background on DNA methylation. Furthermore, we showed that tissue types are important contributors of DNA methylation states.
最近的研究表明，基因表达变化、染色质和 DNA 甲基化受到遗传因素的影响。然而，遗传背景和组织类型对全基因组水平 DNA 甲基化的影响尚未得到广泛描述。为了研究遗传背景和组织类型对全球 DNA 甲基化的影响，我们使用 Affymetrix 500K SNP 阵列对 30 例食管鳞癌（ESCC）患者的肿瘤、相邻正常组织和血液 DNA 进行了 DNA 甲基化分析。利用 30 个人的多种组织，我们能够评估 DNA 甲基化状态在组织和个体之间的变化。我们的结果表明，同一个体的血液和食管组织具有相似的 DNA 甲基化模式，这表明遗传背景对 DNA 甲基化有影响。此外，我们还表明，组织类型是 DNA 甲基化状态的重要贡献者。
