Thérapeutiques Cliniques et Expérimentales des Infections, EA3826, Université de Nantes - INSERM, UFR Médecine, 1 rue Gaston Veil, Nantes, 44000, France.
Microb Ecol. 2010 May;59(4):830-7. doi: 10.1007/s00248-010-9637-2. Epub 2010 Feb 26.
Antibiotic-associated diarrhea (AAD) is associated with altered intestinal microflora and other symptoms that may lead to possibly death. In critically ill patients, diarrhea increases rates of morbimortality. Assessing diarrhea risks is thus important for clinicians. For this reason, we conducted a hypothesis-generating study focused on AAD to provide insight into methods of prevention. We evaluated the hypothesis of predisposing factors within the resident intestinal microbiota in a cohort of outpatients receiving antibiotherapy. Among the pool of tested variables, only those related to bacterial 16S rRNA genes were found to be relevant. Complex statistical analyses provided further information: amid the bacteria 16S rRNA genes, eight were determined to be essential for diarrhea predisposition and characterized from the most important to the least. Using these markers, AAD risk could be estimated with an error of 2%. This molecular analysis offers new perspectives for clinical applications at the level of prevention.
抗生素相关性腹泻（AAD）与肠道微生物群改变和其他可能导致死亡的症状有关。在危重病患者中，腹泻会增加发病率和死亡率。因此，评估腹泻风险对临床医生来说很重要。出于这个原因，我们进行了一项针对 AAD 的假设生成研究，旨在深入了解预防方法。我们在接受抗生素治疗的门诊患者队列中评估了常驻肠道微生物群中易患因素的假设。在测试的变量池中，只有与细菌 16S rRNA 基因相关的变量被认为是相关的。复杂的统计分析提供了更多信息：在细菌 16S rRNA 基因中，有 8 个被确定为腹泻易感性的必需基因，并根据其重要性从最重要到最不重要进行了特征描述。使用这些标志物，AAD 风险的估计误差可以控制在 2%。这种分子分析为预防层面的临床应用提供了新的视角。
