Department of Pediatrics/2501 JCP, University of Iowa Hospital, Iowa City, IA 52242, USA.
Curr Top Microbiol Immunol. 2010;342:99-111. doi: 10.1007/82_2009_5.
ORF47, a serine protein kinase of varicella-zoster virus (VZV) and homolog of herpes simplex virus UL13, is an interesting modulator of VZV pathogenesis. This chapter summarizes research showing that ORF47 protein kinase activity, by virtue of phosphorylation of or binding to various viral substrates, regulates VZV proteins during all phases of viral infection and has a pronounced effect on the trafficking of gE, the predominant VZV glycoprotein, which in turn is critical for cell-to-cell spread of the virus. Casein kinase II, an ubiquitous cellular protein kinase, recognizes a similar but less stringent phosphorylation consensus sequence and can partially compensate for lack of ORF47 activity in VZV-infected cells. Differences between the phosphorylation consensus sites of the viral and cellular kinases are outlined in detail.
ORF47 是水痘带状疱疹病毒（VZV）的丝氨酸蛋白激酶，也是单纯疱疹病毒 UL13 的同源物，是 VZV 发病机制的一个有趣的调节剂。本章总结了研究结果，表明 ORF47 蛋白激酶活性通过磷酸化或结合各种病毒底物，调节病毒感染的所有阶段的 VZV 蛋白，对 gE（主要的 VZV 糖蛋白）的运输有显著影响，gE 反过来对于病毒的细胞间传播至关重要。酪蛋白激酶 II 是一种普遍存在的细胞蛋白激酶，它识别一个类似但不那么严格的磷酸化共有序列，并且可以部分补偿 VZV 感染细胞中 ORF47 活性的缺乏。详细列出了病毒和细胞激酶磷酸化共有序列之间的差异。
