Plastic and Reconstructive Surgery, Hospital General Dr Gea Manuel Gonzalez, Miami, Florida, USA.
J Reconstr Microsurg. 2010 Jul;26(5):335-40. doi: 10.1055/s-0030-1249317. Epub 2010 Feb 25.
Periosteum's role in fracture healing is widely recognized, and its function in bone tissue engineering shows great potential. Here we introduce a novel periosteal free flap to be used as an abundant source of periosteum in the engineering and repair of bone. The descending branch of the lateral femoral circumflex vessels were isolated on 11 fresh human cadavers, preserving perforators to the vastus intermedius muscle. A cuff of vastus intermedius and approximately 75% of the circumference of the femoral periosteum were harvested from 6 cm proximal to the knee to 8 cm distal to the greater trochanter. Flap pedicle length and periosteal dimensions were measured. The pedicle arteries were injected with radiopaque dye, and radiographs were taken. A musculoperiosteal flap was elevated with visible descending perforators in each case. Mean flap surface area was 128 cm(2) (+/-99-143 cm(2)). Average pedicle length was 8 cm (+/-6-11 cm). Dye injection confirmed that the flaps blood supply was the descending branch of the lateral femoral circumflex artery. This anatomical study confirms the vascular supply of this large musculoperiosteal flap. Future work will test its efficacy as an osteoinductive agent in bone repair and tissue engineering in humans.
骨膜在骨折愈合中的作用已被广泛认识，其在骨组织工程中的作用也显示出巨大的潜力。我们在此介绍一种新型的骨膜游离皮瓣，作为骨工程和修复中骨膜的丰富来源。在 11 具新鲜人体尸体上，分离了旋股外侧动脉降支，保留了向股中间肌的穿支。从膝关节近端 6cm 至大转子远端 8cm 处切取股中间肌和大约 75%的股骨骨膜，形成皮瓣。测量皮瓣蒂的长度和骨膜的尺寸。皮瓣蒂的动脉被注入不透射线的染料，然后拍摄 X 线片。在每例中均可见明显的降支穿支，掀起肌骨膜瓣。皮瓣的平均面积为 128cm²（+/-99-143cm²）。蒂的平均长度为 8cm（+/-6-11cm）。染料注射证实皮瓣的血供来源于旋股外侧动脉降支。本解剖学研究证实了这种大型肌骨膜瓣的血供。未来的工作将测试其作为骨修复和组织工程中的成骨诱导剂在人体中的疗效。
