Department of Oral and Maxillofacial Surgery, 521 Parnassus Avenue, C-522, University of California, San Francisco, San Francisco, CA 04143-0440, USA.
Head Neck. 2010 Sep;32(9):1246-68. doi: 10.1002/hed.21358.
Completion of the human genome project approximately 15 years ago was followed closely by advancements in array technology. Investigators quickly applied this new powerful tool to the genomic and proteomic study of oral squamous cell carcinoma (OSCC). Resultant publications documented chromosome, gene, mRNA, and protein alterations that characterize oral cancer. In this review, we summarize how the genomic, proteomic, and epigenetic array studies have provided insight into the process of oral carcinogenesis. We discuss the significant limitations and requirement for validation of these array studies. We also review the manner in which state-of-the-art, high-throughput approaches are being used to search for salivary and serum oral cancer biomarkers.
大约 15 年前，人类基因组计划的完成紧随其后的是阵列技术的进步。研究人员迅速将这一新的强大工具应用于口腔鳞状细胞癌（OSCC）的基因组和蛋白质组研究。由此产生的出版物记录了表征口腔癌的染色体、基因、mRNA 和蛋白质改变。在这篇综述中，我们总结了基因组、蛋白质组和表观基因组阵列研究如何深入了解口腔癌变过程。我们讨论了这些阵列研究的显著局限性和验证要求。我们还回顾了如何使用最先进的高通量方法来寻找唾液和血清口腔癌生物标志物。
