Department of Trauma and Orthopaedic Surgery, Mater Misericordiea University Hospital, Dublin, Ireland.
J Orthop Res. 2010 Sep;28(9):1252-6. doi: 10.1002/jor.21110.
Biomaterial-related infections continue to hamper the success of reconstructive and arthroplasty procedures in orthopaedic surgery. Staphylococci are the most common etiologic agents, with biofilm formation representing a major virulence factor. Biofilms increase bacterial resistance to antimicrobial agents and host immune responses. In staphylococci, production of polysaccharide intercellular adhesin (PIA) by the enzyme products of the icaADBC operon is the best understood mechanism of biofilm development, making the ica genes a potential target for biofilm inhibitors. In this study we report that the antibacterial agent povidone-iodine (PI) also has anti-biofilm activity against Staphylococcus epidermidis and Staphylococcus aureus at sub-inhibitory concentrations (p < 0.001). Inhibition of biofilm by PI correlated with decreased transcription of the icaADBC operon, which in turn correlated with activation of the icaR transcriptional repressor in Staphylococcus epidermidis. These data reveal an additional therapeutic benefit of PI and suggest that studies to evaluate suitability of PI as biomaterial coating agent to reduce device-related infections are merited.
生物材料相关性感染一直阻碍着骨科手术中重建和关节置换手术的成功。葡萄球菌是最常见的病原体，生物膜形成是主要的毒力因素。生物膜增加了细菌对抗生素和宿主免疫反应的抵抗力。在葡萄球菌中，由icaADBC 操纵子的酶产物产生的多糖细胞间黏附素（PIA）是生物膜形成的最佳理解机制，使得 ica 基因成为生物膜抑制剂的潜在靶标。在这项研究中，我们报告了聚维酮碘（PI）在亚抑菌浓度下（p < 0.001）对表皮葡萄球菌和金黄色葡萄球菌也具有抗生物膜活性。PI 对生物膜的抑制作用与 icaADBC 操纵子转录减少相关，而这又与表皮葡萄球菌中 icaR 转录阻遏物的激活相关。这些数据揭示了 PI 的另一种治疗益处，并表明值得进行研究以评估 PI 作为生物材料涂层剂以减少与器械相关感染的适用性。
