Gou Sha, Huang Jun
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
Huan Jing Ke Xue. 2009 Dec;30(12):3645-51.
Heterotrophic nitrification-aerobic granular sludge was successfully formed by batch culture method, with the heterotrophic nitrification bacteria with aerobic denitrification abilities as inoculants, and activated carbon powder as pre-immobilizer. The granulation conditions were studied. Results show that organic loading rate, wastewater characteristics, aeration rate and settling time play important roles in the sludge granulation. Heterotrophic nitrification-aerobic granular sludge can grow on both synthetic wastewater and piggery wastewater. Granulation occurred under the conditions of COD loading rate was 4.0 g x (L x d)(-1), NH4+ -N loading rate was 0.212 g x (L x d)(-1), aeration rate was 200 L x h(-1) and settling time was 2.0-4.0 min. Filamentous bacteria overgrowth was observed when the organic loading rate increasing with synthetic wastewater using glucose as sole carbon resource. It resulted worse settling and denitrification of the aerobic granular sludge. When piggery wastewater was used as influent, the filamentous bacteria were disappeared, better settling and denitrification of the aerobic granular sludge was regained and the granular sludge was grown up. The grown-up granular sludge showed good stability. And its removal rate of TN, NH4+ -N and COD were 84.75%-88.33%, over 99.9% and 97.29%-98.62% respectively when the granular sludge treat piggery wastewater with glucose as additional carbon resource under the loading rates of TN, NH4+ -N and COD were 0.409-0.474 g x (L x d)(-1), 0.285-0.304 g'(L'd)-'and 6.6-8.6 g'(L'd)-' respectively.
采用分批培养法，以具有好氧反硝化能力的异养硝化细菌为接种物，以活性炭粉末为预固定剂，成功形成了异养硝化-好氧颗粒污泥。研究了颗粒化条件。结果表明，有机负荷率、废水特性、曝气速率和沉降时间对污泥颗粒化起着重要作用。异养硝化-好氧颗粒污泥能在合成废水和养猪废水中生长。在COD负荷率为4.0 g·(L·d)⁻¹、NH₄⁺-N负荷率为0.212 g·(L·d)⁻¹、曝气速率为200 L·h⁻¹、沉降时间为2.0 - 4.0 min的条件下发生颗粒化。当以葡萄糖为唯一碳源的合成废水有机负荷率增加时，观察到丝状菌过度生长。这导致好氧颗粒污泥的沉降和反硝化性能变差。当以养猪废水为进水时，丝状菌消失，好氧颗粒污泥恢复了较好的沉降和反硝化性能，颗粒污泥得以生长。生长成熟的颗粒污泥表现出良好的稳定性。当颗粒污泥以葡萄糖为外加碳源处理养猪废水，TN、NH₄⁺-N和COD负荷率分别为0.409 - 0.474 g·(L·d)⁻¹、0.285 - 0.304 g·(L·d)⁻¹和6.6 - 8.6 g·(L·d)⁻¹时，其对TN、NH₄⁺-N和COD的去除率分别为84.75% - 88.33%、超过99.9%和97.29% - 98.62%。
