Ma Heng-liang, Zhan Xin-hua, Zhang Xiao-bin, Zhou Li-xiang
College of Resources and Environment Science, Nanjing Agricultural University, Nanjing 210095, China.
Huan Jing Ke Xue. 2009 Dec;30(12):3684-90.
The dynamic changes of soil enzymatic activities during remediation of phenanthrene contaminated soil with clover or clover and wheat were investigated with pot experiments. Clover and wheat increased the activities of soil sucrase, polyphenol oxidase, urease and phosphatase with an increase rate in the range of 14.72%-46.52%, but inhibited the catalase activity with an inhibition rate in the range of 36.13%-94.79%. Sucrase and polyphenol oxidase reached the maximum activity values at the 14th day, and urease and phosphatase at the 21th day. Catalase got to the minimum activity value at the 7th day. In the light of these, catalase was relatively more sensitive to phenanthrene than the other enzymes, and could be employed as a key indicator to evaluate the risk of polycyclic aromatic hydrocarbon contaminated soil during remedying. Sucrase and polyphenol oxidase activities in wheat and clover intercropping system were significantly higher than in wheat or clover single-cropping system. Furthermore, there was a good negative correlation between catalase and sucrase (r = - 0.482), and polyphenol oxidase (r = -0.599), and urease (r = -0.329), and phosphatase (r = -0.297). Nonetheless, a good positive correlation existed among sucrase, polyphenol oxidase, urease and phosphatase. It is concluded that in the process of phytoremediation, soil enzymes can be employed as indicators for soil quality, it is not necessary to monitor each enzymatic activity, but to the activity of a key enzyme.
通过盆栽试验研究了三叶草或三叶草与小麦修复菲污染土壤过程中土壤酶活性的动态变化。三叶草和小麦提高了土壤蔗糖酶、多酚氧化酶、脲酶和磷酸酶的活性，提高率在14.72% - 46.52%之间，但抑制了过氧化氢酶的活性，抑制率在36.13% - 94.79%之间。蔗糖酶和多酚氧化酶在第14天达到最大活性值，脲酶和磷酸酶在第21天达到最大活性值。过氧化氢酶在第7天达到最小活性值。据此，过氧化氢酶对菲的敏感性相对高于其他酶，可作为评估多环芳烃污染土壤修复过程中风险的关键指标。小麦和三叶草间作系统中的蔗糖酶和多酚氧化酶活性显著高于小麦或三叶草单作系统。此外，过氧化氢酶与蔗糖酶（r = - 0.482）、多酚氧化酶（r = -0.599）、脲酶（r = -0.329）和磷酸酶（r = -0.297）之间存在良好的负相关。然而，蔗糖酶、多酚氧化酶、脲酶和磷酸酶之间存在良好的正相关。研究得出结论，在植物修复过程中，土壤酶可作为土壤质量的指标，无需监测每种酶的活性，只需监测关键酶的活性。
