Department of Clinical Analysis, Hospital Clínico San Carlos, Madrid, Spain.
Rev Esp Enferm Dig. 2010 Jan;102(1):20-31. doi: 10.4321/s1130-01082010000100004.
Angiogenesis plays an important role in tumor progression. The vascular endothelial growth factor (VEGF) is an important regulator of angiogenesis. In the present study we evaluated single nucleotide polymorphisms (SNPs) -2578C > A, -1154G > A, and +936C > T in the VEGF gene, and their prognostic value for patients operated on for colorectal cancer (CRC).
VEGF polymorphisms have been analyzed in 177 patients who had undergone surgical resection at Hospital Clínico San Carlos. The analysis of these polymorphisms was performed with specific probes for each nucleotide in a multiplex reaction using real-time PCR.
We only found a statistically significant relationship for one of these three polymorphisms, +936C > T, with gender and tumor location; 10.7% of patients heterozygotes for this SNP had tumors located in proximal colon, 35.2% in distal segment and 54.1% in rectum (p = 0.03). Patients with the +936T/T genotype had 100% overall survival (OS).
Patients with a +936T/T genotype showed increased survival, therefore the +936C > T SNP could be a useful marker in the follow-up and clinical management of patients with colorectal cancer.
血管生成在肿瘤进展中起着重要作用。血管内皮生长因子（VEGF）是血管生成的重要调节剂。本研究评估了血管内皮生长因子基因中的单核苷酸多态性（SNP）-2578C > A、-1154G > A 和 +936C > T，及其对接受结直肠癌（CRC）手术治疗的患者的预后价值。
对 177 名在圣卡洛斯临床医院接受手术切除的患者进行了 VEGF 多态性分析。使用实时 PCR 进行的多重反应中，对每个核苷酸的特异性探针进行了这些多态性的分析。
我们只发现了这三个多态性中的一个，即 +936C > T，与性别和肿瘤位置有统计学显著关系；携带此 SNP 杂合子的 10.7%的患者肿瘤位于近端结肠，35.2%位于远端节段，54.1%位于直肠（p = 0.03）。+936T/T 基因型的患者总生存率为 100%。
+936T/T 基因型的患者生存率增加，因此 +936C > T SNP 可能是结直肠癌患者随访和临床管理的有用标志物。
