National Taiwan University Hospital, and College of Medicine, Taipei, Taiwan.
Tissue Eng Part C Methods. 2010 Apr;16(2):291-300. doi: 10.1089/ten.tec.2009.0298.
autologous chondrocyte implantation usually requires in vitro cell expansion before implantation. We compared the efficacy of cartilage regeneration by in vitro-expanded chondrocytes at high density and freshly harvested chondrocytes at low density.
surgically created osteochondral defects at weight-bearing surface of femoral condyles of domestic pigs were repaired by biphasic cylindrical porous plugs of DL-poly-lactide-co-glycolide and beta-tricalcium phosphate. Plugs were seeded with autologous chondrocytes in its chondral phase, and press-fit to defects. Seeded cells were (1) in vitro-expanded chondrocytes harvested from stifle joint 3 weeks before implantation and (2) freshly harvested chondrocytes from recipient knee. Seeding densities were 70 x 10(6) and 7 x 10(6) cells/mL, respectively. Cell-free plugs served as control and defects remained untreated as null control. Outcome was examined at 6 months with International Cartilage Repair Society Scale.
the two experimental groups were repaired by hyaline cartilage with collagen type II and Safranin-O. Tissue in control group was primarily fibrocartilage. No regeneration was found in null control. Experimental groups had higher mean International Cartilage Repair Society scores than control in surface, matrix, and cell distribution, but were comparable with control in cell viability, subchondral bone, and mineralization. No significant difference existed between two experimental groups in any of the six categories. Uni-axial indentation test revealed similar creeping stress-relaxation property as native cartilage on experimental, but not control, specimen.
cartilage could regenerate in both experimental models, in comparable quality. Culture of chondrocytes before implantation is not necessary.
自体软骨细胞植入术通常需要在植入前进行体外细胞扩增。我们比较了体外高密度扩增的软骨细胞和低浓度新鲜收获的软骨细胞在软骨再生方面的疗效。
采用 DL-聚乳酸-共-乙醇酸和β-磷酸三钙的双相柱状多孔塞修复家猪股骨髁负重面的手术性骨软骨缺损。软骨相的塞子接种了自体软骨细胞，并采用压配的方式植入缺损处。接种的细胞为（1）植入前 3 周从膝关节中收获的体外扩增软骨细胞，和（2）来自受者膝关节的新鲜收获的软骨细胞。接种密度分别为 70×106 和 7×106 个细胞/ml。无细胞塞子作为对照，缺损处未做任何处理作为空白对照。在 6 个月时采用国际软骨修复协会评分标准进行评估。
两组实验均通过 II 型胶原和番红 O 再生出透明软骨。对照组的组织为纤维软骨。空白对照未发现再生。实验组的国际软骨修复协会评分在表面、基质和细胞分布方面均高于对照组，但在细胞活力、软骨下骨和矿化方面与对照组相当。在六个类别中，实验组之间无显著差异。单轴压痕试验表明，实验标本具有与天然软骨相似的蠕动应力松弛特性，但对照组则没有。
两种实验模型均能再生出具有可比性质量的软骨。植入前培养软骨细胞并非必需。
