激动型抗 CD40 单克隆抗体 5C11 通过 CD40 信号转导对胃癌细胞生物学行为的影响。
CD40 signaling activated by agonistic anti-CD40 monoclonal antibody 5C11 has different effects on biological behavior of gastric carcinoma cells.
机构信息
Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Key Laboratory of Medicine and Clinical Immunology of Jiangsu Province, Suzhou 215006, China.
出版信息
Immunol Lett. 2010 Jul 8;131(2):120-5. doi: 10.1016/j.imlet.2010.02.007. Epub 2010 Feb 25.
A gastric cancer cell line, AGS has high-level expression of CD40. CD40 is present on the surfaces of a large variety of cells, including B cells, endothelial cells, dendritic cells and some carcinoma cells, and delivers signals regulating diverse cellular responses, such as proliferation, differentiation, growth suppression, cell death. In this research, the expression of CD40 and CD40 transcription in gastric cancer cell lines (AGS, BGC-823, HGC-27, SGC-7901) was investigated by semi-quantitative RT-PCR. Cancer cell proliferation inhibitory assay was also performed using different concentrations of anti-CD40 monoclonal antibody (mAb), 5C11. Results indicated that treatment of 5C11 alone and combined 5C11, IFN-alpha and 5-FU both significantly inhibited cancer cell proliferation. The synergistic effects of combined 5C11, IFN-alpha and 5-FU on growth inhibition of human gastric cancer AGS cells could be observed. These results suggest that humanized anti-CD40 monoclonal antibody (mAb), 5C11 can be used as a valuable reagent for clinical application.
AGS 是一株高表达 CD40 的胃癌细胞系。CD40 存在于多种细胞表面，包括 B 细胞、内皮细胞、树突状细胞和某些癌细胞，传递调节多种细胞反应的信号，如增殖、分化、生长抑制、细胞死亡。在这项研究中，通过半定量 RT-PCR 研究了胃癌细胞系（AGS、BGC-823、HGC-27、SGC-7901）中 CD40 和 CD40 转录的表达。还使用不同浓度的抗 CD40 单克隆抗体（mAb）5C11 进行了癌细胞增殖抑制测定。结果表明，单独使用 5C11 以及联合使用 5C11、IFN-α和 5-FU 均可显著抑制癌细胞增殖。联合使用 5C11、IFN-α和 5-FU 对人胃癌 AGS 细胞生长抑制的协同作用可以观察到。这些结果表明，人源化抗 CD40 单克隆抗体（mAb）5C11 可用作临床应用的有价值试剂。
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