Department of Chemistry and Environmental Science, Fujian Province University Key Laboratory of Analytical Science, Zhangzhou Normal University, Zhangzhou 363000, China.
Talanta. 2010 Apr 15;81(1-2):162-6. doi: 10.1016/j.talanta.2009.11.052. Epub 2009 Dec 2.
A novel protein molecularly imprinted membrane (PMIM) was synthesized on the surface of multiwalled carbon nanotubes (MWNTs) through a surface molecular imprinting technique by using bovine serum albumin (BSA) as the template molecule, acrylamide (AAm) as the functional monomer, N,N'-methylenebisacrylamide (NNMBA) as the cross-linker and ammonium persulphate [(NH(4))(2)S(2)O(8)] as the initiator. The amounts of raw materials were optimized in this paper and the suitable amount is 0.1g of CNTs, 0.02 g of BSA, 0.24 g of AAm, 0.1g of MBA and 0.025 mg of (NH(4))(2)S(2)O(8). The selective recognition ability of PMIM/MWNTs was evaluated using adsorption experiments. Maximum adsorption capacity was 5.53 microg/mg PMIM/MWNTs and a saturation value was achieved at a BSA concentration of 0.2mg/mL. The selectivity adsorption experiments showed that the PMIM/MWNTs also had higher adsorption capacities for BSA than for such molecules, as HSA, HB, pepsin and HRP. The PMIM/MWNTs displayed a 2.6-fold increase in affinity to BSA compared to the nPMIM/MWNTs. The PMIM/MWNTs, on the other hand, did not exhibit any significant change in affinity to other molecules compared to the nPMIM/MWNTs. In the experiment, the characteristics of the PMIM/MWNTs were analyzed using infrared spectroscopy and their configurations were observed using scanning electron microscopy.
一种新型的蛋白质分子印迹膜(PMIM)通过表面分子印迹技术在多壁碳纳米管(MWNTs)表面合成,以牛血清白蛋白(BSA)为模板分子,丙烯酰胺(AAm)为功能单体,N,N'-亚甲基双丙烯酰胺(NNMBA)为交联剂,过硫酸铵[(NH(4))(2)S(2)O(8)]为引发剂。本文优化了原料用量,适宜用量为 0.1g CNTs、0.02g BSA、0.24g AAm、0.1g MBA 和 0.025mg [(NH(4))(2)S(2)O(8)]。通过吸附实验评价了 PMIM/MWNTs 的选择性识别能力。最大吸附容量为 5.53μg/mg PMIM/MWNTs,在 BSA 浓度为 0.2mg/mL 时达到饱和值。选择性吸附实验表明,PMIM/MWNTs 对 BSA 的吸附容量也高于 HSA、HB、胃蛋白酶和 HRP 等分子。与 nPMIM/MWNTs 相比,PMIM/MWNTs 对 BSA 的亲和力提高了 2.6 倍。另一方面,与 nPMIM/MWNTs 相比,PMIM/MWNTs 对其他分子的亲和力没有明显变化。在实验中,采用红外光谱分析了 PMIM/MWNTs 的特性,并用扫描电子显微镜观察了其结构。