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在多壁碳纳米管表面制备和特性的蛋白质分子印迹膜。

Preparation and characteristics of protein molecularly imprinted membranes on the surface of multiwalled carbon nanotubes.

机构信息

Department of Chemistry and Environmental Science, Fujian Province University Key Laboratory of Analytical Science, Zhangzhou Normal University, Zhangzhou 363000, China.

出版信息

Talanta. 2010 Apr 15;81(1-2):162-6. doi: 10.1016/j.talanta.2009.11.052. Epub 2009 Dec 2.

Abstract

A novel protein molecularly imprinted membrane (PMIM) was synthesized on the surface of multiwalled carbon nanotubes (MWNTs) through a surface molecular imprinting technique by using bovine serum albumin (BSA) as the template molecule, acrylamide (AAm) as the functional monomer, N,N'-methylenebisacrylamide (NNMBA) as the cross-linker and ammonium persulphate [(NH(4))(2)S(2)O(8)] as the initiator. The amounts of raw materials were optimized in this paper and the suitable amount is 0.1g of CNTs, 0.02 g of BSA, 0.24 g of AAm, 0.1g of MBA and 0.025 mg of (NH(4))(2)S(2)O(8). The selective recognition ability of PMIM/MWNTs was evaluated using adsorption experiments. Maximum adsorption capacity was 5.53 microg/mg PMIM/MWNTs and a saturation value was achieved at a BSA concentration of 0.2mg/mL. The selectivity adsorption experiments showed that the PMIM/MWNTs also had higher adsorption capacities for BSA than for such molecules, as HSA, HB, pepsin and HRP. The PMIM/MWNTs displayed a 2.6-fold increase in affinity to BSA compared to the nPMIM/MWNTs. The PMIM/MWNTs, on the other hand, did not exhibit any significant change in affinity to other molecules compared to the nPMIM/MWNTs. In the experiment, the characteristics of the PMIM/MWNTs were analyzed using infrared spectroscopy and their configurations were observed using scanning electron microscopy.

摘要

一种新型的蛋白质分子印迹膜(PMIM)通过表面分子印迹技术在多壁碳纳米管(MWNTs)表面合成,以牛血清白蛋白(BSA)为模板分子,丙烯酰胺(AAm)为功能单体,N,N'-亚甲基双丙烯酰胺(NNMBA)为交联剂,过硫酸铵[(NH(4))(2)S(2)O(8)]为引发剂。本文优化了原料用量,适宜用量为 0.1g CNTs、0.02g BSA、0.24g AAm、0.1g MBA 和 0.025mg [(NH(4))(2)S(2)O(8)]。通过吸附实验评价了 PMIM/MWNTs 的选择性识别能力。最大吸附容量为 5.53μg/mg PMIM/MWNTs,在 BSA 浓度为 0.2mg/mL 时达到饱和值。选择性吸附实验表明,PMIM/MWNTs 对 BSA 的吸附容量也高于 HSA、HB、胃蛋白酶和 HRP 等分子。与 nPMIM/MWNTs 相比,PMIM/MWNTs 对 BSA 的亲和力提高了 2.6 倍。另一方面,与 nPMIM/MWNTs 相比,PMIM/MWNTs 对其他分子的亲和力没有明显变化。在实验中,采用红外光谱分析了 PMIM/MWNTs 的特性,并用扫描电子显微镜观察了其结构。

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