Sapienza Università di Roma, Dipartimento di Chimica e Tecnologie del Farmaco, Piazzale Aldo Moro 5, 00185 Roma, Italy.
Talanta. 2010 Apr 15;81(1-2):235-40. doi: 10.1016/j.talanta.2009.11.063. Epub 2009 Dec 3.
In this work we have developed and characterized the use of Laccases from Trametes versicolor (TvL) and Trametes hirsuta (ThL) as biocatalytic components of electrochemical biosensors for the determination of polyphenol index in wines. Polyazetidine prepolimer (PAP) was used as immobilizing agent, multi-walled and single-walled carbon nanotubes screen-printed electrodes as sensors (MWCNTs-SPE and SWCNTs-SPE) and gallic acid as standard substrate. The amperometric measurements were carried out by using a flow system at a fixed potential of -100 mV vs. silver/silver chloride electrode in Britton-Robinson buffer 0.1 mol L(-1), pH 5. The results were compared with those obtained with the Folin-Ciocalteau reference method. The results obtained in the analysis of twelve Italian wines put in evidence the better suitability of ThL-MWCNTs-based biosensor in the determination of the polyphenol index in wines. This biosensor shows fast and reliable amperometric responses to gallic acid with a linear range 0.1-18.0 mg L(-1) (r(2)=0.999). The influence of the interferences on both spectrophotometric and electrochemical measurements have been carefully evaluated.
在这项工作中，我们开发并表征了来自彩绒革盖菌（TvL）和粗毛栓菌（ThL）的漆酶的用途，将其作为电化学生物传感器的生物催化元件，用于测定葡萄酒中的多酚指数。聚氮杂环戊烯预聚物（PAP）被用作固定化剂，多壁和单壁碳纳米管丝网印刷电极作为传感器（MWCNTs-SPE 和 SWCNTs-SPE），没食子酸作为标准底物。在 Britton-Robinson 缓冲液 0.1 mol L(-1)，pH 5 中，在固定电位-100 mV 对银/氯化银电极进行安培测量，通过使用流动系统进行。结果与福林-希奥考尔特参考方法获得的结果进行了比较。对 12 种意大利葡萄酒进行的分析结果表明，基于 ThL-MWCNTs 的生物传感器在葡萄酒中多酚指数的测定中具有更好的适用性。该生物传感器对没食子酸具有快速可靠的安培响应，线性范围为 0.1-18.0 mg L(-1)（r(2)=0.999）。对分光光度法和电化学测量的干扰的影响进行了仔细评估。
