一种用于筛选谷物和玉米基婴儿食品中 T-2 和 HT-2 毒素的快速光学免疫分析方法。
A rapid optical immunoassay for the screening of T-2 and HT-2 toxin in cereals and maize-based baby food.
机构信息
Institute of Agri-food and Land Use, School of Biological Sciences, Queen's University of Belfast, David Keir Building, Stranmillis Road, Belfast BT9 5AG, United Kingdom.
出版信息
Talanta. 2010 Apr 15;81(1-2):630-6. doi: 10.1016/j.talanta.2009.12.055. Epub 2010 Jan 11.
A rapid surface plasmon resonance (SPR) screening assay has been developed for the combined detection of T-2 and HT-2 toxins in naturally contaminated cereals using a sensor chip coated with an HT-2 toxin derivative and a monoclonal antibody. The antibody raised against HT-2 displayed high cross-reactivity with T-2 toxin while there was no cross-reaction observed with other commonly occurring trichothecenes. A simple extraction procedure using 40% methanol was applied to baby food, breakfast cereal, and wheat samples prior to biosensor analysis. Limits of detection (LOD) for each matrix were determined as 25 microg kg(-1) for baby food and breakfast cereal and 26 microg kg(-1) for wheat. Intra-assay precision (n=6) was calculated for each matrix. The results were expressed as the relative standard deviation and determined as 2.8% (100 microg kg(-1)) and 1.8% (200 microg kg(-1)) in breakfast cereal, 4.6% (50 microg kg(-1)) and 3.6% (100 microg kg(-1)) in wheat and 0.97% (25 microg kg(-1)) and 6.3% (50 microg kg(-1)) in baby food. Between run precision (n=3) performed at the same levels yielded relative standard deviations of 6.7% and 3.9% for breakfast cereals, 3.3% and 1.6% for wheat and 6.8% and 0.08% for baby food, respectively.
一种快速表面等离子体共振（SPR）筛选分析方法已经被开发出来，用于同时检测天然污染谷物中的 T-2 和 HT-2 毒素，该方法使用涂覆有 HT-2 毒素衍生物和单克隆抗体的传感器芯片。针对 HT-2 产生的抗体与 T-2 毒素显示出高度的交叉反应性，而与其他常见的单端孢霉烯没有观察到交叉反应。在生物传感器分析之前，使用 40%甲醇对婴儿食品、早餐麦片和小麦样品进行了简单的提取程序。每个基质的检测限（LOD）确定为婴儿食品和早餐麦片为 25μgkg(-1)，小麦为 26μgkg(-1)。每个基质的日内精密度（n=6）均进行了计算。结果表示为相对标准偏差，早餐麦片为 2.8%（100μgkg(-1))和 1.8%（200μgkg(-1))，小麦为 4.6%（50μgkg(-1))和 3.6%（100μgkg(-1))，婴儿食品为 0.97%（25μgkg(-1))和 6.3%（50μgkg(-1))。在相同水平下进行的运行间精密度（n=3）分别产生了早餐麦片为 6.7%和 3.9%、小麦为 3.3%和 1.6%、婴儿食品为 6.8%和 0.08%的相对标准偏差。
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