肿瘤坏死因子-α（--308）和-β（252）基因多态性与妊娠期糖尿病发病的关系。

Association between polymorphisms in human tumor necrosis factor-alpha (--308) and -beta (252) genes and development of gestational diabetes mellitus.

机构信息

Department of Obstetrics and Gynaecology, Faculty of Medicine and Health, International Medical University, 126, Jalan 19/155B, Bukit Jalil, 57000 Kuala Lumpur, Malaysia.

出版信息

Diabetes Res Clin Pract. 2010 May;88(2):139-45. doi: 10.1016/j.diabres.2010.01.028. Epub 2010 Feb 26.

DOI:10.1016/j.diabres.2010.01.028

PMID:20189261

Abstract

OBJECTIVE

The aim of this study is to investigate if an association exists between single nucleotide polymorphism (SNP) in the tumor necrosis factor-alpha (TNF-alpha) and TNF-beta genes.

METHODS

The DNA was extracted and SNP in the human TNF-alpha and TNF-beta genes at positions -308 (G/A) and 252 (A/G), respectively, was analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Plasma levels of TNF-alpha in different stages of pregnancy were quantified using enzyme linked immunosorbent assay (ELISA).

RESULTS

There was no significant difference in genotype and allele frequency of SNP at position -308 (G/A) in the promoter region of the human TNF-alpha gene as well as the SNP at position 252 (A/G) in the human TNF-beta gene between the GDM and control subjects. Using the logistic regression model, it was found that the SNP in the TNF-alpha as well as TNF-beta were not associated with development of GDM. In addition, the TNF-alpha levels in the plasma of GDM and control mothers were not significantly different.

CONCLUSIONS

In the population studied, the SNP in position -308 (G/A) of the human TNF-alpha or in position 252 (A/G) of the human TNF-beta gene is not an independent risk factor or a predictor for GDM.

摘要

目的

本研究旨在探讨肿瘤坏死因子-α（TNF-α）和 TNF-β 基因中单核苷酸多态性（SNP）之间是否存在关联。

方法

采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，提取 DNA，分析人 TNF-α 和 TNF-β 基因在位置-308（G/A）和 252（A/G）的 SNP。采用酶联免疫吸附试验（ELISA）定量检测不同妊娠阶段孕妇血浆中 TNF-α 的水平。

结果

在 TNF-α 基因启动子区位置-308（G/A）的 SNP 以及 TNF-β 基因位置 252（A/G）的 SNP 中，GDM 组和对照组的基因型和等位基因频率均无显著差异。采用逻辑回归模型发现，TNF-α 和 TNF-β 的 SNP 与 GDM 的发生无关。此外，GDM 组和对照组母亲血浆中的 TNF-α 水平无显著差异。