Michikawa M, Kobayashi T, Tsukagoshi H
Department of Neurology, Tokyo Medical and Dental University, Japan.
Brain Res. 1991 Jan 4;538(1):79-85. doi: 10.1016/0006-8993(91)90379-a.
Early events of chemical transmission were examined in our newly established heterotypic co-culture system with human muscle in monolayer and fetal rat spinal cord explants with attached dorsal root ganglia. The mean amplitude, frequency, rise time and half-fall time of miniature end-plate potentials (MEPPs) were 1.04 mV, 3.9/s, 6.1 ms and 54.9 ms, respectively. Time intervals between successive MEPPs were exponentially distributed. Stimulus evoked potentials were successfully obtained. The distribution of stimulus evoked end-plate potential (EPP) amplitudes was approximately equal to a Poisson distribution. This is the first report concerning intracellular recordings of chemical transmission of developing neuromuscular junctions in heterotypic co-culture system using human muscle. In this co-culture system, the heterotypic neuromuscular junctions show significant similarities previously to those observed in nerve-muscle tissues co-cultured from the same species as well as immature nerve-muscle synapses in situ.
在我们新建立的异型共培养系统中，对化学传递的早期事件进行了研究，该系统包含单层培养的人肌肉以及带有背根神经节的胎鼠脊髓外植体。微小终板电位（MEPPs）的平均幅度、频率、上升时间和半下降时间分别为1.04 mV、3.9/s、6.1 ms和54.9 ms。连续MEPPs之间的时间间隔呈指数分布。成功获得了刺激诱发电位。刺激诱发的终板电位（EPP）幅度分布近似于泊松分布。这是关于在使用人肌肉的异型共培养系统中发育中的神经肌肉接头化学传递的细胞内记录的首次报告。在这个共培养系统中，异型神经肌肉接头与在同物种共培养的神经肌肉组织以及原位未成熟神经肌肉突触中观察到的情况此前显示出显著相似性。
