Department of Food Hygiene and Technology, University of Kirikkale, Faculty of Veterinary Medicine, 71450 Yahsihan, Kirikkale, Turkey.
Res Vet Sci. 2010 Oct;89(2):153-8. doi: 10.1016/j.rvsc.2010.02.005. Epub 2010 Mar 1.
The prevalence of enterococci in neck skin samples of poultry from Ankara region in Turkey was investigated and their antibiotic resistance patterns were determined. In the study, 83 of 106 analyzed neck skin samples were positive for Enterococcus, with E. faecium as the most prevalent species (48%) followed by E. durans (23%) and E. faecalis (19%). Lower numbers were detected for E. gallinarum, E. hirae, E. mundtii and E. casseliflavus. Using the disc diffusion method, it was established that over 90% of E. faecium and E. faecalis isolates were high-level resistant against erythromycin and tetracycline. Four E. faecium isolates were additionally resistant to chloramphenicol, gentamicin and streptomycin, though they were susceptible to penicillin G. The most frequently observed multiple resistance in E. faecium (25%) was against erythromycin, tetracycline, chloramphenicol and streptomycin. Of the E. faecalis isolates, 44% were multiple resistant to erythromycin, tetracycline and streptomycin. Vancomycin resistance could not be demonstrated phenotypically and vanA or vanB genes were not detected by multiplex PCR in any of the isolates. Nevertheless, the observed resistance patterns are of concern for public health.
本研究调查了土耳其安卡拉地区家禽颈部皮肤样本中肠球菌的流行情况，并确定了它们的抗生素耐药模式。在这项研究中，106 个分析的颈部皮肤样本中有 83 个为肠球菌阳性，其中屎肠球菌（48%）最为常见，其次是耐久肠球菌（23%）和粪肠球菌（19%）。鸡肠球菌、海氏肠球菌、屎肠球菌和坚韧肠球菌的检出率较低。采用纸片扩散法发现，超过 90%的屎肠球菌和粪肠球菌分离株对红霉素和四环素呈高水平耐药。有 4 株屎肠球菌分离株对氯霉素、庆大霉素和链霉素耐药，尽管它们对青霉素 G 敏感。屎肠球菌最常见的多重耐药（25%）是对红霉素、四环素、氯霉素和链霉素。44%的粪肠球菌分离株对红霉素、四环素和链霉素呈多重耐药。表型上未检测到万古霉素耐药性，也未通过多重 PCR 在任何分离株中检测到 vanA 或 vanB 基因。然而，所观察到的耐药模式令人关注。
