Life Sciences Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen, PR China.
Chemosphere. 2010 Apr;79(5):595-604. doi: 10.1016/j.chemosphere.2010.01.052. Epub 2010 Mar 1.
The contamination of marine ecosystems by endocrine disrupting compounds (EDCs) is of great concern. Protein expression profile maybe a good method to help us understand the molecular mechanisms of EDCs-toxicity to aquatic organisms. In this study, the abalone (Haliotis diversicolor supertexta), was selected as the target organism. Toxicological effects of two reference endocrine disruptors: diallyl phthalate (DAP, 50microgL(-1)) and bisphenol-A (BPA, 100microgL(-1)) were investigated after a three months static-renewal exposure on abalones using proteomics to analyze their hepatopancreas tissues. Some enzyme activity parameters of hepatopancreas extracts were also performed, including Na(+)-K(+)-ATPase, Ca(2+)-Mg(2+)-ATPase, peroxidase (POD) and malondialdehyde (MDA) production. After analyzing the proteomics profile of hepatopancreas by 2D gel electrophoresis, we found that 24 spots significantly increased or decreased at protein expression level (2-fold difference) in the 2D-maps from the treatment groups. Eighteen out of 24 protein spots were successfully identified by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-MS). These proteins can be roughly categorized into diverse functional classes such as detoxification, oxidative stress, hormone regulating, cellular metabolism and innate immunity. In addition, the enzymatic results indicated that DAP/BPA exposure affected the oxidative stress status and the cellular homeostasis, which partly corroborated the proteomics' results. Taken together, these data demonstrate that proteomics is a powerful tool to provide valuable insights into possible mechanisms of toxicity of EDCs contaminants in aquatic species. Additionally, the results highlight the potential of abalone as a valuable candidate for investigating EDCs impacts on marine ecosystems.
海洋生态系统受到内分泌干扰化合物（EDCs）的污染引起了极大关注。蛋白质表达谱可能是一种很好的方法，可以帮助我们了解 EDCs 对水生生物毒性的分子机制。在这项研究中，鲍鱼（Haliotis diversicolor supertexta）被选为研究对象。采用蛋白质组学技术分析其肝胰腺组织，研究了两种参考内分泌干扰物：邻苯二甲酸二烯丙酯（DAP，50μg/L）和双酚 A（BPA，100μg/L）在鲍鱼身上经过三个月的静态更新暴露后的毒性效应。还对肝胰腺提取物的一些酶活性参数进行了测定，包括 Na(+)-K(+)-ATP 酶、Ca(2+)-Mg(2+)-ATP 酶、过氧化物酶（POD）和丙二醛（MDA）的产生。通过 2D 凝胶电泳分析肝胰腺的蛋白质组学图谱后，我们发现处理组 2D 图谱中蛋白质表达水平（2 倍差异）显著增加或减少的有 24 个斑点。24 个蛋白质斑点中的 18 个通过基质辅助激光解吸/电离串联飞行时间质谱（MALDI-TOF-MS）成功鉴定。这些蛋白质大致可分为解毒、氧化应激、激素调节、细胞代谢和先天免疫等多种功能类别。此外，酶学结果表明，DAP/BPA 暴露影响了氧化应激状态和细胞内稳态，这部分印证了蛋白质组学的结果。综上所述，这些数据表明蛋白质组学是一种强大的工具，可以为了解 EDCs 污染物对水生生物毒性的可能机制提供有价值的见解。此外，这些结果强调了鲍鱼作为研究 EDCs 对海洋生态系统影响的有价值候选物种的潜力。
