Research Center of Environmental Engineering and Management, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, People's Republic of China.
Ecotoxicology. 2011 Aug;20(6):1233-45. doi: 10.1007/s10646-011-0672-7. Epub 2011 Apr 9.
Acute toxic effects and mechanisms of two typical endocrine disrupting chemicals, nonylphenols (NPs) and bisphenol A (BPA), to the embryonic development of the abalone Haliotis diversicolor supertexta, were investigated by the two-stage embryo toxicity test. The 12-h median effective concentrations (EC(50)) of NPs and BPA to the trochophore development were 1016.22 and 30.72 μg L(-1), respectively, and the respective 96-h EC(50) values based on the completion of metamorphosis (another experimental endpoint) were reduced to 11.65 and 1.02 μg L(-1). Longer exposure time and magnified exposure concentrations in the benthic diatom, that serves as both food source and settlement substrate during the metamorphosis, via bioaccumulation, led to the higher sensitivity of metamorphosis to target EDCs compared with the trochophore development. The hazard concentrations for 5% of the species (HC(5)) could be employed as the safety thresholds for the embryonic development of the abalone. The 12-h HC(5) values of NPs and BPA were 318.68 and 13.93 μg L(-1), respectively, and the respective 96-h HC(5) values were 0.99 and 0.18 μg L(-1), which were at environmentally relevant levels. Results of proteomic responses revealed that NPs and BPA altered various functional proteins in the abalone larvae with slight differences between each chemical and affected various physiological functions, such as energy and substance metabolism, cell signalling, formation of cytoskeleton and cilium, immune and stress responses at the same time, leading to the failure of metamorphosis.
采用两阶段胚胎毒性试验研究了两种典型的内分泌干扰化学物质壬基酚（NPs）和双酚 A（BPA）对厚壳贻贝幼虫胚胎发育的急性毒性效应及作用机制。NP 和 BPA 对担轮幼虫发育的 12 h 半数有效浓度（EC50）分别为 1016.22 和 30.72 μg/L，基于变态完成的 96 h EC50 值分别降低至 11.65 和 1.02 μg/L。在变态过程中，底栖硅藻既是贻贝幼虫的食物来源，也是附着基质，通过生物蓄积作用，延长暴露时间和提高暴露浓度，导致变态对目标 EDCs 的敏感性高于担轮幼虫发育。物种危害浓度的 5%（HC5）可作为贻贝胚胎发育的安全阈值。NP 和 BPA 的 12 h HC5 值分别为 318.68 和 13.93 μg/L，96 h HC5 值分别为 0.99 和 0.18 μg/L，均处于环境相关水平。蛋白质组学反应结果表明，NP 和 BPA 改变了贻贝幼虫的各种功能蛋白，两种化学物质之间略有差异，同时影响了能量和物质代谢、细胞信号转导、细胞骨架和纤毛形成、免疫和应激反应等各种生理功能，导致变态失败。
