酒精性肌病：维生素 D 缺乏与小鼠模型中肌纤维萎缩有关。

Alcoholic myopathy: vitamin D deficiency is related to muscle fibre atrophy in a murine model.

机构信息

Departamento de Medicina Interna, Hospital Universitario, Ofra s/n 38320 La Laguna, Tenerife, Canary Islands, Spain.

出版信息

Alcohol Alcohol. 2010 May-Jun;45(3):223-30. doi: 10.1093/alcalc/agq010. Epub 2010 Feb 26.

DOI:10.1093/alcalc/agq010

PMID:20190231

Abstract

AIMS

Chronic myopathy has been described in alcoholics, characterized by atrophy of type II fibres, and vitamin D deficiency. Low serum vitamin D levels are frequent in alcoholics. The possibility exists that serum vitamin D levels are related to muscle changes in a murine experimental model.

METHODS

Histological analysis of the right gastrocnemius muscle was performed in four groups of adult Sprague-Dawley rats, sacrificed after 5 weeks of treatment following the Lieber-DeCarli model. We studied the association between muscle histological changes and the activity of glutathione peroxidase (GPX), superoxide dismutase (SOD) and lipid peroxidation products (malondialdehyde); parathyroid hormone (PTH), insulin-like growth factor 1 (IGF-1), free testosterone, 1,25 dihydroxyvitamin D3 (vitamin D) and corticosterone; and serum calcium and magnesium.

RESULTS

Alcoholic animals showed type IIa and IIb fibre atrophy, especially the low-protein-fed ones, an effect dependent on protein deficiency. A significant relationship was observed between serum vitamin D levels and IIa fibre area (rho = 0.56, P = 0.002), and also, as a trend, between vitamin D and type IIb fibre area (rho = 0.39, p = 0.053); between vitamin D and muscle GPX (rho = 0.40, P = 0.025) and SOD activities (rho = 0.43, P = 0.012). Muscle GPX activity was significantly related with type I fibre area (rho = 0.49, P = 0.01) and muscle SOD, with type IIa fibre area (rho = 0.38, P = 0.045). Serum testosterone was also related with type IIa fibre area (rho = 0.61, P < 0.001). No relation was observed between serum PTH, corticosterone, or IGF-1 and fibre area PTH and antioxidant systems. Multiple regression analysis disclosed that the only parameter independently related with type IIa fibre area was serum vitamin D.

CONCLUSION

Low vitamin D levels are related to muscle fibre atrophy, and altered levels of muscle antioxidant enzymes could play a role in alcoholic myopathy.

摘要

目的

慢性肌病在酗酒者中已有描述，其特征为 II 型纤维萎缩和维生素 D 缺乏。酗酒者的血清维生素 D 水平往往较低。在一个鼠类实验模型中，血清维生素 D 水平与肌肉变化之间存在相关性的可能性是存在的。

方法

对 4 组成年 Sprague-Dawley 大鼠右侧腓肠肌进行组织学分析，这些大鼠在采用 Lieber-DeCarli 模型进行 5 周治疗后被处死。我们研究了肌肉组织学变化与谷胱甘肽过氧化物酶 (GPX)、超氧化物歧化酶 (SOD) 和脂质过氧化产物 (丙二醛)；甲状旁腺激素 (PTH)、胰岛素样生长因子 1 (IGF-1)、游离睾酮、1,25 二羟维生素 D3 (维生素 D) 和皮质酮；以及血清钙和镁之间的关联。

结果

酒精中毒动物表现出 IIa 和 IIb 型纤维萎缩，尤其是低蛋白喂养的动物，这种效应依赖于蛋白质缺乏。血清维生素 D 水平与 IIa 纤维面积呈显著相关（rho = 0.56，P = 0.002），并且，呈趋势相关，与 IIb 纤维面积也呈趋势相关（rho = 0.39，p = 0.053）；与肌肉 GPX（rho = 0.40，P = 0.025）和 SOD 活性（rho = 0.43，P = 0.012）也呈趋势相关。肌肉 GPX 活性与 I 型纤维面积显著相关（rho = 0.49，P = 0.01），与肌肉 SOD 也显著相关，与 IIa 纤维面积相关（rho = 0.38，P = 0.045）。血清睾酮也与 IIa 纤维面积相关（rho = 0.61，P < 0.001）。血清 PTH、皮质酮或 IGF-1 与纤维面积之间无相关性，PTH 和抗氧化系统也无相关性。多元回归分析显示，与 IIa 型纤维面积独立相关的唯一参数是血清维生素 D。