Anhui Province Key Laboratory of R&D of Chinese Medicine, Anhui Key Laboratory of Modernized Chinese Material, Anhui University of Traditional Chinese Medicine, China.
Biol Pharm Bull. 2010;33(3):415-20. doi: 10.1248/bpb.33.415.
Although anticancer effect of gambogic acid (GA) and its potential mechanisms were well documented in past decades, limited information is available on the anticancer effect of gambogenic acid (GNA), another major active component of Gamboge. Here we performed a study to determine whether GNA possesses anticancer effect and find its potential mechanisms. The results suggested that GNA significantly inhibited the proliferation of several tumor cell lines in vitro and in vivo. Treatment with GNA dose and time dependently induced A549 cells apoptosis, arrested the cells to G0/G1 phase in vitro and down-regulated the expression of cyclin D1 and cyclooxygenase (COX)-2 in mRNA level. In addition, anticancer effect was further demonstrated by applying xenografts in nude mice coupled with the characteristic of apoptosis in the GNA treated group. Taken together, these observations might suggest that GNA inhibits tumor cell proliferation via apoptosis-induction and cell cycle arrest.
虽然藤黄酸（GA）在过去几十年中的抗癌作用及其潜在机制已经得到了很好的证明，但关于藤黄酸（GNA）的抗癌作用及其潜在机制的信息却很有限，GNA 是藤黄的另一种主要活性成分。在这里，我们进行了一项研究，以确定 GNA 是否具有抗癌作用及其潜在机制。结果表明，GNA 能显著抑制体外和体内多种肿瘤细胞系的增殖。用 GNA 处理能剂量和时间依赖性地诱导 A549 细胞凋亡，在体外将细胞阻滞于 G0/G1 期，并下调 cyclin D1 和环氧化酶（COX）-2 的 mRNA 水平。此外，在裸鼠异种移植中进一步证明了抗癌作用，并结合了 GNA 处理组的凋亡特征。总之，这些观察结果可能表明 GNA 通过诱导细胞凋亡和细胞周期阻滞来抑制肿瘤细胞增殖。
